首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人血小板因子4(PF-4/CXCL4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PF4含量。PF4即血小板因子4，是血小板α-颗粒分泌的一种特异性蛋白。它在炎症、免疫调节及肿瘤微环境等方面都发挥重要作用。研究机制上，其可通过与受体相互作用，参与细胞趋化、增殖及血管生成等过程，为相关疾病的治疗提供潜在靶点。试剂盒检测范围为6.25 ng/mL-400 ng/mL，灵敏度为1.56 ng/mL。适用于血小板活化相关机制研究、心血管疾病模型分析、肿瘤微环境生物标志物探索等科研领域，支持体外实验中不同来源样本（如血液或组织提取物）的PF - 4水平检测，为疾病病理机制解析及药物干预效果评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：C-X-C motif chemokine 4 ELISA Kit; Chemokine (C X C motif) ligand 4 ELISA Kit; Chemokine (CXC motif) ligand 4 ELISA Kit; chemokine ligand 4 ELISA Kit; CXCL 4 ELISA Kit; CXCL4 ELISA Kit; Iroplact ELISA Kit; MGC138298 ELISA Kit; Oncostatin A ELISA Kit; Oncostatin-A ELISA Kit; OncostatinA ELISA Kit; PF 4 ELISA Kit; PF-4 ELISA Kit; PF4 ELISA Kit; Pf4a ELISA Kit; Platelet factor 4 ELISA Kit; PLF4_HUMAN ELISA Kit; SCYB 4 ELISA Kit; SCYB4 ELISA Kit; short form ELISA Kit; Small inducible cytokine subfamily B ELISA Kit; Small inducible cytokine subfamily member 4 ELISA Kit
缩写：PF4
Uniprot No.：P02776
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 92-101 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 83-99 | |||||
| 1:80 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 86-97 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 4002.5482.4522.5002.294 2002.1962.0762.1361.930 1001.6321.5931.6131.407 501.0821.1221.1020.896 250.6750.6450.6600.454 12.50.4410.4360.4390.233 6.250.3200.3150.3180.112 00.2050.2070.206 | ||||||
货期：3-5 working days
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