Human Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit

产品描述：人血血小板衍生生长因子AB(PDGF-AB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PDGF-AB含量。PDGF-AB是一种由血小板分泌的生长因子，由A和B链组成，参与调控纤维母细胞和平滑肌细胞等中胚层来源细胞的增殖和迁移。其研究机制涉及PDGF与PDGFRα和PDGFRβ受体的结合，激活下游信号通路，促进细胞增殖和血管生成。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于体外实验中对PDGF-AB的定量分析，例如探究心血管疾病模型中的信号通路机制、评估药物干预对细胞因子表达的影响，或通过组织样本分析疾病相关分子标志物变化，为细胞生物学、病理学及药理学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。

缩写：PDGF-AB

种属：Homo sapiens (Human)

样本类型：serum, plasma, tissue homogenates

检测范围：31.25 pg/mL-2000 pg/mL

灵敏度：7.8 pg/mL

反应时间：1-5h

样本体积：50-100ul

检测波长：450 nm

研究领域：Cancer

测定原理：quantitative

测定方法：Sandwich

精密度：

 Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.  

线性度：

 To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

回收率：

 The recovery of human PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

标准曲线：

 These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

货期：3-5 working days