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产品描述：人类粘蛋白2(ORM2)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ORM2含量。试剂盒检测范围为78 ng/mL-5000 ng/mL，灵敏度为19.5 ng/mL。适用于科研领域中对ORM2蛋白表达水平的定量分析，例如探索炎症相关疾病机制、评估代谢综合征生物标志物或研究肝脏功能异常模型中的蛋白动态变化本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ORM2 ELISA Kit; AGP2Alpha-1-acid glycoprotein 2 ELISA Kit; AGP 2 ELISA Kit; Orosomucoid-2 ELISA Kit; OMD 2 ELISA Kit
缩写：ORM2
Uniprot No.：P19652
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ORM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 98 | ||||
| Range % | 90-102 | |||||
| 1:200 | Average % | 89 | ||||
| Range % | 83-94 | |||||
| 1:400 | Average % | 101 | ||||
| Range % | 93-105 | |||||
| 1:800 | Average % | 105 | ||||
| Range % | 97-110 | |||||
回收率：
| The recovery of human ORM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 82-95 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 50000.1310.1350.133 25000.3890.4010.395 12500.7500.7320.741 6251.0421.0201.031 3121.4411.4541.448 1561.6501.6661.658 781.8061.7671.787 02.0012.0782.040 | ||||||
货期：3-5 working days
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