Mouse soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1(sLOX-1)ELISA Kit

中文名： 小鼠可溶性凝集素样氧化低密度脂蛋白受体1(sLOX-1)ELISA Kit

货号: IPD-E15846m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠可溶性凝集素样氧化低密度脂蛋白受体1(sLOX-1)酶联免疫试剂盒）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的OLR1含量。OLR1是一种C型凝集素超家族的低密度脂蛋白受体，通过环状AMP信号通路调控，参与结合、内化并降解氧化的低密度脂蛋白，可能影响动脉粥样硬化、心肌梗死和阿尔茨海默病风险。其突变与多种疾病相关，是重要的研究靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。本产品适用于心血管疾病动物模型研究、内皮细胞功能调控机制探索、药物干预效果评估等科研领域，能够为sLOX - 1在氧化应激相关疾病中的分子机制研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Olr1 ELISA Kit; Lox1 ELISA Kit; Oxidized low-density lipoprotein receptor 1 ELISA Kit; Ox-LDL receptor 1 ELISA Kit; Lectin-like oxidized LDL receptor 1 ELISA Kit; LOX-1 ELISA Kit; Lectin-like oxLDL receptor 1 ELISA Kit; Lectin-type oxidized LDL receptor 1) [Cleaved into: Oxidized low-density lipoprotein receptor 1 ELISA Kit; soluble form] ELISA Kit
缩写：
OLR1
Uniprot No.：
Q9EQ09
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)650.940626.003659.296668.871640.230648.557SD0.0400.0380.0330.0490.0520.046CV(%)5.5255.4054.5146.6317.2736.371

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse sLOX-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-93
1:2	Average %	98
	Range %	90-102
1:4	Average %	98
	Range %	91-101
1:8	Average %	98
	Range %	91-102

回收率：

The recovery of mouse sLOX-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-95
EDTA plasma (n=4)	99	91-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.592	2.487	2.540	2.414
2500	1.837	1.876	1.857	1.731
1250	1.159	1.123	1.141	1.015
625	0.766	0.745	0.756	0.630
312	0.403	0.422	0.413	0.287
156	0.273	0.258	0.266	0.140
78	0.211	0.218	0.215	0.089
0	0.127	0.125	0.126