Recombinant Human MVD protein

中文名： 二磷酸戊酸脱羧酶(MVD)重组蛋白
别名： MVD;MPD;Diphosphomevalonate decarboxylase

货号: PA1000-2046

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>85%SDS-PAGE.
种属	Human
靶点	MVD
Uniprot No	P53602
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-400aa
氨基酸序列	MGSSHHHHHHSSGLVPRGSHMASEKPLAAVTCTAPVNIAVIKYWGKRDEE LVLPINSSLSVTLHQDQLKTTTTAVISKDFTEDRIWLNGREEDVGQPRLQ ACLREIRCLARKRRNSRDGDPLPSSLSCKVHVASVNNFPTAAGLASSAAG YACLAYTLARVYGVESDLSEVARRGSGSACRSLYGGFVEWQMGEQADGKD SIARQVAPESHWPELRVLILVVSAEKKLTGSTVGMRASVETSPLLRFRAE SVVPARMAEMARCIRERDFPSFAQLTMKDSNQFHATCLDTFPPISYLNAI SWRIIHLVHRFNAHHGDTKVAYTFDAGPNAVIFTLDDTVAEFVAAVWHGF PPGSNGDTFLKGLQVRPAPLSAELQAALAMEPTPGGVKYIIVTQVGPGPQ ILDDPCAHLLGPDGLPKPAA
预测分子量	46 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于MVD(甲羟戊酸脱羧酶，Mevalonate decarboxylase)重组蛋白研究的模拟参考文献示例(建议通过学术数据库如PubMed、Google Scholar检索最新真实文献)：

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1. **文献名称**: "Heterologous Expression and Characterization of Recombinant Mevalonate Decarboxylase in *E. coli*"

**作者**: Zhang Y, et al.

**摘要**: 本研究报道了通过大肠杆菌表达系统克隆并纯化重组MVD蛋白，优化了表达条件并验证了其催化甲羟戊酸转化为异戊烯焦磷酸(IPP)的酶活性，为类异戊二烯代谢工程提供基础。

2. **文献名称**: "Structural Insights into Mevalonate Decarboxylase via Recombinant Protein Crystallography"

**作者**: Lee S, Kim T.

**摘要**: 通过重组MVD蛋白的结晶和X射线衍射分析，揭示了其三维结构及关键活性位点，阐明了底物结合与催化机制，为针对胆固醇合成通路的药物设计提供依据。

3. **文献名称**: "Enhancing Isoprenoid Production via Engineering of Recombinant MVD in Saccharomyces cerevisiae"

**作者**: Voynova NE, et al.

**摘要**: 在酿酒酵母中过表达重组MVD蛋白，结合代谢通量分析，显著提高了萜类化合物产量，证明了MVD在合成生物学中的调控作用。

4. **文献名称**: "Functional Analysis of Mutant MVD Recombinant Proteins in Familial Hypercholesterolemia"

**作者**: Patel R, et al.

**摘要**: 构建了与遗传性高胆固醇血症相关的MVD突变体重组蛋白，体外实验表明部分突变导致酶活性丧失，揭示了疾病发生的分子机制。

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**注意**：以上文献为模拟示例，实际研究需查阅具体数据库。推荐使用关键词“Mevalonate decarboxylase recombinant”、“MVD protein expression”或结合研究领域(如代谢工程、酶催化)进一步检索。

背景信息

**Background of MVD Recombinant Protein**

MVD (mevalonate diphosphate decarboxylase) is a critical enzyme in the mevalonate pathway, a metabolic route essential for synthesizing isoprenoids, including cholesterol, steroid hormones, and nonsteroidal isoprenoid compounds. This pathway is conserved across eukaryotes and some prokaryotes, playing a central role in cellular metabolism. MVD catalyzes the ATP-dependent decarboxylation of mevalonate-5-diphosphate (MVAPP) to isopentenyl diphosphate (IPP), a key precursor for isoprenoid biosynthesis.

The recombinant form of MVD is produced via genetic engineering techniques, typically by cloning the MVD gene into expression vectors (e.g., bacterial, yeast, or mammalian systems) to enable large-scale protein production. Recombinant MVD retains the enzymatic activity of its native counterpart, allowing researchers to study its structure, kinetics, and regulatory mechanisms in vitro. Its production is vital for drug discovery, particularly in targeting diseases linked to dysregulated cholesterol synthesis, such as cardiovascular disorders or certain cancers.

Interest in MVD also stems from its potential role in microbial metabolism. In pathogens like *Mycobacterium tuberculosis*, the mevalonate pathway is absent, but alternative pathways involving MVD homologs may exist, making it a potential target for antimicrobial agents. Additionally, recombinant MVD is used in metabolic engineering to optimize IPP production for industrial applications, including biofuel and terpenoid-based pharmaceutical synthesis.

Structural studies of recombinant MVD have revealed insights into its catalytic mechanism and allosteric regulation, aiding in the design of inhibitors or modulators. Overall, MVD recombinant protein serves as a versatile tool for both basic research and biotechnological applications, bridging gaps between metabolic understanding and therapeutic innovation.