Bovine Albumin(Alb) ELISA kit

中文名： 牛白蛋白(ALB)ELISA kit

货号: IPD-E08664b

Price： ￥3000

48T 96T

数量：

大包装询价

产品详情

产品描述：
牛白蛋白(ALB)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ALB含量。ALB（白蛋白）是一种在人体血液中广泛存在的蛋白质，主要功能是调节血浆胶体渗透压，并作为多种内源分子的载体。研究显示，ALB在肝癌治疗中具有重要作用，特别是在ALB基因低表达型肝癌中，通过激活ALB基因可以有效抑制肝癌细胞的增殖。其研究机制涉及检测肝癌样株中ALB的表达水平，识别低表达肝癌株，并利用ALB激动剂进行治疗。试剂盒检测范围为0.313 μg/mL-80 μg/mL，灵敏度为0.148 μg/mL。该产品主要用于科研领域，例如牛源性生物制品的质量控制、疫苗或抗体生产过程中血清蛋白的监测，以及畜牧研究中牛只健康状态评估等场景，为蛋白表达水平研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
ALB ELISA Kit; Serum albumin ELISA Kit; BSA ELISA Kit; allergen Bos d 6 ELISA Kit
缩写：
ALB
Uniprot No.：
P02769
种属：
Bos taurus (Bovine)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.313 μg/mL-80 μg/mL
灵敏度：
0.148 μg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Metabolism
测定原理：
quantitative
测定方法：
Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(μg/ml)	5.265	5.989	5.270	5.475	5.510	4.853
SD	0.038	0.033	0.038	0.041	0.044	0.048
CV(%)	6.463	5.546	6.179	6.732	7.237	7.643

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Bovine Alb in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2000

Average %

Range %

89-113

1:4000

Average %

Range %

86-107

1:8000

Average %

Range %

85-108

1:16000

Average %

Range %

83-98

回收率：

The recovery of Bovine Alb spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	93	88-95
EDTA plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.





μg/ml	OD1	OD2	Average
80	0.309	0.289	0.299
20	0.443	0.423	0.433
5	0.579	0.568	0.573
1.25	0.857	0.848	0.853
0.313	1.504	1.498	1.501
0	2.242	2.238	2.240