首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人N端前脑钠素(NT-proBNP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NT-proBNP含量。NT-proBNP是一种重要的生物标志物。其背景在于它是脑钠肽原经蛋白酶切割后的无活性片段，水平变化与心室壁压力和容量负荷密切相关。研究机制上，常通过检测血液中它的含量，辅助诊断心力衰竭、评估预后及指导治疗。试剂盒检测范围为0.313 ng/ml-20 ng/ml，灵敏度为0.216 ng/mL。适用于心血管疾病机制研究、动物模型评估或体外实验中NT - proBNP表达水平的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：NT-proBNP
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.313 ng/ml-20 ng/ml
灵敏度：0.216 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
An assay plate --- The 96-well plate is composed of 12 x 8 strip plates and can be taken apart.
Two bottles of lyophilized standard --- Reconstitute one bottle of the standard with sample diluent at dilution series and draw the standard curve.
Biotin-labeled NT-proBNP antibody (100 x concentrate) 1 x 120 μl --- Act as the detection antibody and need to be diluted before use.
HRP-avidin (100 x concentrate) 1 x 120 μl --- Catalyze the TMB substrate for color development and need to be diluted before use.
Biotin-antibody Diluent 1 x 15 ml --- Dilute the Biotin-antibody.
HRP-avidin Diluent 1 x 15 ml --- Dilute the HRP-avidin.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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