人NOV蛋白同源物(NOV)酶联免疫试剂盒

中文名： Human Protein NOV homolog(NOV) ELISA kit

货号: IPD-EL015956HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人NOV蛋白同源物(NOV)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOV含量。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。可用于研究者定量分析不同样本中NOV蛋白的表达水平。通过检测该蛋白在生理或病理状态下的浓度变化，可用于探索其在胚胎发育、器官损伤修复或癌症进展中的分子作用机制，为相关疾病的分子调控网络研究提供实验工具，适用于分子生物学、细胞生物学等基础研究领域。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CCN family member 3 ELISA Kit; CCN3 ELISA Kit; IBP-9 ELISA Kit; IGF-binding protein 9 ELISA Kit; IGFBP-9 ELISA Kit; IGFBP9 ELISA Kit; Insulin-like growth factor-binding protein 9 ELISA Kit; Nephroblastoma overexpressed ELISA Kit; Nephroblastoma overexpressed gene protein homolog ELISA Kit; Nephroblastoma-overexpressed gene protein homolog ELISA Kit; NOV ELISA Kit; NOV_HUMAN ELISA Kit; NovH ELISA Kit; Protein NOV homolog ELISA Kit
缩写：
NOV
Uniprot No.：
P48745
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
62.5 pg/mL-4000 pg/mL
灵敏度：
15.6 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)507.598498.924515.234498.924508.687502.170SD0.0350.0410.0400.0500.0480.045CV(%)4.9165.8245.5637.1026.7326.365

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NOV in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:5

Average %

Range %

95-110

1:10

Average %

103

Range %

99-112

1:20

Average %

Range %

88-98

1:40

Average %

Range %

93-104

回收率：

The recovery of human NOV spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
4000	2.522	2.496	2.509	2.392
2000	1.692	1.642	1.667	1.550
1000	1.110	1.094	1.102	0.985
500	0.713	0.689	0.701	0.584
250	0.434	0.401	0.418	0.301
125	0.347	0.353	0.350	0.233
62.5	0.208	0.205	0.207	0.090
0	0.120	0.114	0.117