Human Neurogenic locus notch homolog protein 1(NOTCH1) ELISA kit

中文名： 人跨膜受体蛋白Notch-1(NOTCH1)ELISA kit

货号: IPD-EL015949HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人跨膜受体蛋白Notch-1(NOTCH1)酶联免疫试剂盒）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NOTCH1含量。NOTCH1是一种在多细胞生物体中高度保守的细胞信号转导系统，参与细胞分化、增殖、凋亡和个体发育。其研究机制涉及Notch受体与配体相互作用，通过γ-分泌酶裂解释放胞内结构域，进入细胞核激活下游靶基因。在肿瘤发生发展中，NOTCH1信号通路异常激活与多种肿瘤的发生发展密切相关。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于基础科研领域中对Notch-1信号通路的功能研究，例如细胞间通讯实验、疾病模型构建或药物干预效果评估等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
hN1 ELISA Kit; Neurogenic locus Notch homolog protein 1 ELISA Kit; NICD ELISA Kit; NOTC1_HUMAN ELISA Kit; Notch 1 ELISA Kit; Notch 1 intracellular domain ELISA Kit; Notch homolog 1 translocation associated ELISA Kit; notch1 ELISA Kit; TAN1 ELISA Kit; Translocation associated Notch protein TAN1 ELISA Kit; Translocation-associated notch protein TAN-1 ELISA Kit
缩写：
NOTCH1
Uniprot No.：
P46531
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Epigenetics and Nuclear Signaling
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)631.041602.691606.472587.560598.909629.151SD0.0250.0210.0260.0290.0330.031CV(%)5.1444.4595.4976.2637.0366.392

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human NOTCH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

94-101

1:4

Average %

Range %

89-99

1:8

Average %

Range %

89-97

1:16

Average %

Range %

82-92

回收率：

The recovery of Human NOTCH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	96	88-102
EDTA plasma (n=4)	89	83-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.426	2.614	2.520	2.375
2500	1.508	1.423	1.466	1.321
1250	0.784	0.825	0.805	0.660
625	0.496	0.477	0.487	0.342
312	0.309	0.317	0.313	0.168
156	0.255	0.264	0.260	0.115
78	0.197	0.192	0.195	0.050
0	0.144	0.145	0.145