Human Nidogen 1 (NID1) ELISA Kit

中文名： 人巢蛋白1(NID1) ELISA Kit

货号: IPD-E13289h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人巢蛋白1(NID1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的NID1含量。试剂盒检测范围为0.39 ng/mL-25 ng/mL，灵敏度为0.09 ng/mL。适用于基础科研中探索NID1在肿瘤微环境调控、纤维化疾病进程或再生医学中的作用机制，也可用于药物开发模型中评估靶向细胞外基质的干预效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Enactin ELISA Kit; Ent ELISA Kit; Entactin 1 ELISA Kit; Entactin ELISA Kit; NID 1 ELISA Kit; NID ELISA Kit; NID-1 ELISA Kit; Nid1 ELISA Kit; NID1_HUMAN ELISA Kit; Nidogen (enactin) ELISA Kit; Nidogen 1 ELISA Kit; Nidogen ELISA Kit; Nidogen-1 ELISA Kit
缩写：
NID1
Uniprot No.：
P14543
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, cell culture supernates
检测范围：
0.39 ng/mL-25 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Neuroscience
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)3.0463.3712.9173.2873.0393.399SD0.0380.0350.040.0550.0490.057CV(%)4.4553.8854.8026.1875.7516.298

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human NID 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

82-93

1:2

Average %

Range %

94-100

1:4

Average %

Range %

90-104

1:8

Average %

Range %

82-93

回收率：

The recovery of human NID 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-95
EDTA plasma (n=4)	101	96-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











ng/ml	OD1	OD2	Average	Corrected
25	2.186	2.140	2.163	2.016
12.5	1.642	1.633	1.638	1.491
6.25	1.251	1.220	1.236	1.089
3.12	0.873	0.830	0.852	0.705
1.56	0.579	0.541	0.560	0.413
0.78	0.332	0.342	0.337	0.190
0.39	0.260	0.265	0.263	0.116
0	0.148	0.145	0.147