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产品描述：人金属硫蛋白(MT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、尿液、唾液样本中的MT含量。试剂盒检测范围为6.25 pg/mL-400 pg/mL，灵敏度为0.813 pg/mL。适用于科研领域中对MT蛋白表达动态的探究，例如重金属暴露模型的毒性机制研究、氧化应激相关信号通路解析、疾病动物模型或细胞实验中MT的调控规律验证等。试剂盒兼容多种样本类型，为跨体系、多维度实验设计提供灵活支持，助力基础研究中对MT生理功能及分子机制的深入探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CES 1 ELISA Kit; CES1 ELISA Kit; Metallothionein 1A ELISA Kit; Metallothionein 1S ELISA Kit; Metallothionein 2 ELISA Kit; Metallothionein 2A ELISA Kit; Metallothionein IA ELISA Kit; Metallothionein II ELISA Kit; Metallothionein-1A ELISA Kit; Metallothionein-IA ELISA Kit; Metallothionein2 ELISA Kit; MGC32848 ELISA Kit; MT 1A ELISA Kit; MT 2 ELISA Kit; MT 2A ELISA Kit; MT IA ELISA Kit; MT II ELISA Kit; MT-1A ELISA Kit; MT-IA ELISA Kit; MT1 ELISA Kit; MT1A ELISA Kit; MT1A_HUMAN ELISA Kit; MT1S ELISA Kit; MT2 ELISA Kit; MT2A ELISA Kit; MTC ELISA Kit
缩写：MT
Uniprot No.：P04731
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates, urine, saliva
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：0.813 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human MT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MT antibody.
Six vials standard (5 ml/bottle) ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial HRP-conjugated MT antibody (6 ml/bottle) ---Bind to the MT in the samples or standards and react with the substrate to make the solution chromogenic.
Substrate A (1 x 7 ml)
Substrate B (1 x 7 ml)
One vial Stop Solution (7ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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