Human macrophage stimulating protein,MSP ELISA Kit

中文名： 人巨噬细胞刺激蛋白(MSP)ELISA Kit

货号: IPD-E09062h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人巨噬细胞刺激蛋白(MSP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MST1含量。MST1（哺乳动物不育系20样激酶1）是一种在哺乳动物中与酵母Ste20激酶同源的蛋白激酶，作为Hippo抑制通路的核心组件，在果蝇发育调控中起关键作用。MST1通过调节细胞增殖、凋亡和器官大小等，在多种生理和病理过程中发挥重要作用。近期研究发现，MST1在结直肠癌中表达降低，与不良预后相关，可能成为结直肠癌治疗的潜在靶点。试剂盒检测范围为0.156 ng/mL-10 ng/mL，灵敏度为0.039 ng/mL。适用于探索MSP在免疫调节相关疾病模型中的作用机制，如肿瘤侵袭转移研究、纤维化病理进程分析或炎症反应动态监测，为科研人员提供稳定可靠的实验工具。助力于基础医学研究与分子机制解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
D3F15S2 ELISA Kit; DNF15S2 ELISA Kit; Hepatocyte growth factor like protein alpha chain ELISA Kit; Hepatocyte growth factor like protein ELISA Kit; Hepatocyte growth factor like protein beta chain ELISA Kit; Hepatocyte growth factor like protein homolog ELISA Kit; Hepatocyte growth factor-like protein beta chain ELISA Kit; HGFL ELISA Kit; HGFL_HUMAN ELISA Kit; Macrophage stimulating 1 (hepatocyte growth factor like) ELISA Kit; Macrophage stimulatory protein ELISA Kit; Macrophage-stimulating protein ELISA Kit; MSP ELISA Kit; MST1 ELISA Kit; NF15S2 ELISA Kit; OTTHUMP00000208927 ELISA Kit
缩写：
MST1
Uniprot No.：
P26927
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.156 ng/mL-10 ng/mL
灵敏度：
0.039 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(ng/ml)	1.255	1.265	1.297	1.287	1.286	1.317
SD	0.038	0.043	0.035	0.044	0.044	0.053
CV(%)	4.822	5.409	4.294	5.439	5.446	6.401

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MSP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:20

Average %

101

Range %

94-108

1:40

Average %

105

Range %

99-109

1:80

Average %

Range %

82-96

1:160

Average %

Range %

87-99

回收率：

The recovery of human MSP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	104	97-113
EDTA plasma (n=4)	100	95-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



ng/ml	OD1	OD2	Average	Corrected
10	2.827	2.787	2.807	2.704
5	2.314	2.254	2.284	2.181
2.5	1.511	1.566	1.539	1.436
1.25	0.745	0.773	0.759	0.656
0.625	0.412	0.402	0.407	0.304
0.312	0.250	0.242	0.246	0.143
0.156	0.167	0.171	0.169	0.066
0	0.101	0.105	0.103