Human Matrix metalloproteinase 8/Neutrophil collagenase,MMP-8 ELISA kit

中文名： 人基质金属蛋白酶8/中性粒细胞胶原酶(MMP-8)ELISA Kit

货号: IPD-E04680h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人基质金属蛋白酶8/中性粒细胞胶原酶(MMP-8)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP8含量。MMP8，即基质金属蛋白酶8，是一种降解细胞外基质（ECM）的酶，参与多种生理和病理过程。研究发现，MMP8在压力易感性和抑郁症中发挥重要作用，其表达增加与应激相关神经精神疾病的发生发展密切相关。研究机制表明，MMP8通过影响神经元功能和行为，以及改变细胞外空间，参与应激相关障碍的发生。试剂盒检测范围为39.06 pg/mL-2500 pg/mL，灵敏度为9.77 pg/mL。适用于基础研究领域，如炎症性疾病机制探索、纤维化进程分析或肿瘤微环境中基质重塑相关研究，为体外实验提供可靠的蛋白表达水平数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
CLG 1 ELISA Kit; CLG1 ELISA Kit; Collagenase 1 ELISA Kit; Collagenase 1 neutrophil ELISA Kit; HNC ELISA Kit; Matrix metallopeptidase 8 (neutrophil collagenase) ELISA Kit; Matrix metalloprotease 8 ELISA Kit; Matrix metalloproteinase-8 ELISA Kit; MMP 8 ELISA Kit; MMP-8 ELISA Kit; Mmp8 ELISA Kit; MMP8_HUMAN ELISA Kit; Neutrophil collagenase ELISA Kit; PMNL CL ELISA Kit; PMNL collagenase ELISA Kit; PMNL-CL ELISA Kit; PMNLCL ELISA Kit
缩写：
MMP8
Uniprot No.：
P22894
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
39 pg/mL-2500 pg/mL
灵敏度：
9.75 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)313.254310.763312.423312.423311.592314.302SD0.0430.0480.0410.0520.0570.054CV(%)4.4895.0424.2895.4395.9755.619

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

86-99

1:3

Average %

Range %

91-101

1:9

Average %

Range %

89-95

1:27

Average %

Range %

88-98

回收率：

The recovery of human MMP-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	95	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.













pg/ml	OD1	OD2	Average	Corrected
2500	2.580	2.482	2.531	2.403
1250	2.152	2.092	2.122	1.994
625	1.613	1.588	1.601	1.473
312	0.930	0.920	0.925	0.797
156	0.502	0.492	0.497	0.369
78	0.347	0.337	0.342	0.214
39	0.222	0.218	0.220	0.092
0	0.128	0.127	0.128