Human Matrix metalloproteinase 7,MMP-7 ELISA kit

中文名： 人基质金属蛋白酶7(MMP-7)ELISA Kit

货号: IPD-E04679h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人基质金属蛋白酶7(MMP-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP7含量。MMP7 即基质金属蛋白酶 7，在肿瘤发生、发展及转移等过程中扮演重要角色。其研究机制主要聚焦于它对细胞外基质的降解作用，可破坏组织屏障，促进肿瘤细胞的迁移与侵袭，也是潜在的肿瘤诊断和治疗靶点，相关研究持续深入。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于体外研究场景，如肿瘤微环境分析、疾病相关分子机制探索、药物干预效果评估或生物标志物筛选等科研领域，为分子生物学、免疫学及转化医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Matrilysin ELISA Kit; Matrin ELISA Kit; Matrix Metalloproteinase 7 ELISA Kit; Matrix metalloproteinase-7 ELISA Kit; MMP 7 ELISA Kit; MMP-7 ELISA Kit; MMP7 ELISA Kit; MMP7_HUMAN ELISA Kit; MPSL1 ELISA Kit; PUMP 1 ELISA Kit; Pump 1 protease ELISA Kit; Pump-1 protease ELISA Kit; PUMP1 ELISA Kit; Uterine matrilysin ELISA Kit; Uterine metalloproteinase ELISA Kit
缩写：
MMP7
Uniprot No.：
P09237
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)626.781621.749625.103627.619625.942623.426SD0.0410.0390.0420.0540.0580.056CV(%)5.0934.8815.2306.7007.2146.991

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

104

Range %

100-108

1:2

Average %

Range %

84-92

1:4

Average %

Range %

85-98

1:8

Average %

Range %

88-96

回收率：

The recovery of human MMP-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-101
Heparin plasma (n=4)	93	89-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
5000	2.715	2.625	2.670	2.526
2500	2.156	2.249	2.203	2.059
1250	1.435	1.384	1.410	1.266
625	0.860	0.799	0.830	0.686
312	0.498	0.467	0.483	0.339
156	0.289	0.271	0.280	0.136
78	0.215	0.211	0.213	0.069
0	0.145	0.142	0.144