Mouse luteinizing hormone (LH) ELISA kit

货号: IPD-E12770m

Price： ￥2500

48T 96T

数量：

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产品描述：
小鼠黄体生成素（LH）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测样本中的LH含量。LH即促黄体生成素，是腺垂体分泌的糖蛋白激素。其可刺激睾丸间质细胞合成和分泌雄激素，在女性体内可促进排卵和黄体生成。研究机制主要围绕其与下丘脑-垂体-性腺轴的反馈调节，对生殖内分泌功能举足轻重。该产品适用于科研领域的小鼠生殖生理研究，例如卵巢功能评估、激素分泌调控实验或环境因素对生殖轴影响的机制探索，为动物模型中的生殖健康研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
LhbLutropin subunit beta ELISA kit; Lutropin beta chain ELISA kit; Luteinizing hormone subunit beta ELISA kit; LH-B ELISA kit; LSH-B ELISA kit; LSH-beta ELISA kit
缩写：
LH
Uniprot No.：
O09108
种属：
Mus musculus (Mouse)
样本类型：
检测范围：
灵敏度：
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich
本试剂盒所含材料：

A micro ELISA plate --- The 96-well plate has been pre-coated with anti-mouse LH antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Six vials lyophilized standard (0.5 ml/bottle)--- Dilute a bottle ofthe standard at dilution series, read the OD values, and then draw a standard curve.
Biotin-labeled LH antibody 1 x 6 ml---Act as the detection antibody.
HRP-avidin 1 x 11ml --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
Substrate A 1 x 7 ml --- Chromogenic agent A acts as a hydrogen donor.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

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