首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠血管内皮细胞生长因子受体2(VEGFR-2/Flk-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的VEGFR-2/Flk-1含量。VEGFR-2（血管内皮生长因子受体-2）是VEGF（血管内皮生长因子）的主要受体，在血管生成和肿瘤生长中发挥关键作用。其研究机制涉及VEGF与VEGFR-2结合后激活下游信号通路，如Ras/Raf/MAPK和PI3K/Akt，进而影响细胞增殖、存活和迁移。VEGFR-2抑制剂已成为抗肿瘤治疗的重要靶点。试剂盒检测范围为7.8 pg/mL-500 pg/mL，灵敏度为1.95 pg/mL。适用于生命科学研究中血管生成相关机制探讨、抗血管生成药物筛选、肿瘤模型或心血管疾病模型中VEGFR - 2表达水平的动态监测等实验场景，为研究血管生物学及病理生理过程提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Kdr ELISA Kit; Flk-1 ELISA Kit; Flk1 ELISA Kit; Vascular endothelial growth factor receptor 2 ELISA Kit; VEGFR-2 ELISA Kit; EC 2.7.10.1 ELISA Kit; Fetal liver kinase 1 ELISA Kit; FLK-1 ELISA Kit; Kinase NYK ELISA Kit; Protein-tyrosine kinase receptor flk-1 ELISA Kit; CD antigen CD309 ELISA Kit
缩写：KDR
Uniprot No.：P35918
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGFR-2/Flk-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:100 | Average % | 86 | ||
| Range % | 82-91 | |||
| 1:200 | Average % | 101 | ||
| Range % | 98-105 | |||
| 1:400 | Average % | 87 | ||
| Range % | 84-91 | |||
| 1:800 | Average % | 100 | ||
| Range % | 95-105 |
回收率：
| The recovery of mouse VEGFR-2/Flk-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 89 | 85-94 | |||||
| EDTA plasma (n=4) | 107 | 103-110 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 5002.5642.5362.5502.438 2501.5131.5311.5221.410 1250.9850.9610.9730.861 62.50.5510.5600.5560.444 31.20.3450.3510.3480.236 15.60.2420.2510.2470.135 7.80.1740.1760.1750.063 00.1130.1100.112 | |||||||
货期：3-5 working days
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