Human soluble interleukin-2 receptor,IL-2sRα ELISA kit

中文名： 人白介素2可溶性受体α链(IL-2sRα/CD25)ELISA Kit

货号: IPD-E04629h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人白介素2可溶性受体α链(IL-2sRα/CD25)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL2RA含量。IL2RA 是一个重要靶点。它是白细胞介素-2 受体的 α 链，在免疫细胞激活与增殖中发挥关键作用。研究围绕其与配体结合机制展开，旨在通过干预二者结合，调节免疫反应，为免疫相关疾病如自身免疫病、器官移植排斥反应等治疗提供思路。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。该产品特别适用于免疫学基础研究、疾病生物标志物筛选及药物治疗效果评估等科研场景，例如通过检测外周血样本评估系统性红斑狼疮患者的免疫激活状态，或分析肿瘤微环境中免疫调节因子的动态变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
IL2RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD antigen CD25
缩写：
IL2RA
Uniprot No.：
P01589
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)638.872632.048630.909637.736635.463633.187SD0.0310.0260.0340.0460.0440.045CV(%)5.1504.3625.7147.6557.3467.540

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-2sRα in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

86-98

1:2

Average %

101

Range %

92-104

1:4

Average %

Range %

85-94

1:8

Average %

Range %

91-101

回收率：

The recovery of human IL-2sRα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	89-104
EDTA plasma (n=4)	98	91-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



pg/ml	OD1	OD2	Average	Corrected
5000	2.987	2.842	2.915	2.811
2500	2.100	2.212	2.156	2.052
1250	1.217	1.199	1.208	1.104
625	0.582	0.566	0.574	0.470
312	0.339	0.354	0.347	0.243
156	0.225	0.221	0.223	0.119
78	0.157	0.148	0.153	0.049
0	0.105	0.103	0.104