首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素22(IL-22)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL22含量。IL22即白细胞介素-22，由多种免疫细胞产生。其在炎症、组织修复等生理病理过程中发挥作用。研究机制上，IL22通过与受体结合激活下游信号通路，参与调节细胞增殖、分化及炎症因子释放等，在免疫相关疾病研究中受关注。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。科研人员可将其应用于免疫调节机制研究、炎症动物模型评估、感染性疾病模型构建等领域，特别适用于肠道类器官实验、皮肤创伤修复研究或呼吸道感染模型中IL - 22动态变化的监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il22 ELISA Kit; Il22a ELISA Kit; Iltif ELISA Kit; IltifaInterleukin-22 ELISA Kit; IL-22 ELISA Kit; IL-10-related T-cell-derived-inducible factor ELISA Kit; IL-TIF ELISA Kit; IL-TIF alpha ELISA Kit; Interleukin-22a ELISA Kit; IL-22a ELISA Kit
缩写：IL22
Uniprot No.：Q9JJY9
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 106 | ||||
| Range % | 101-113 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 90-98 | |||||
回收率：
| The recovery of mouse IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 89 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 202.3092.4382.3742.157 101.6281.7291.6791.462 51.1931.2211.2070.990 2.50.8240.8050.8150.598 1.250.6050.6270.6160.399 0.6250.4620.4500.4560.239 0.3120.3270.3120.3200.103 00.2200.2140.217 | ||||||
货期：3-5 working days
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