首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠白介素1受体拮抗剂(IL1Ra)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1RN含量。IL1RN作为靶点，它与炎症调节密切相关。研究发现，IL1RN可以竞争性结合IL-1受体，从而抑制IL-1的生物学活性。这种作用机制使其在炎症相关疾病的治疗研究中备受关注，为开发新型抗炎药物提供了重要方向。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，灵敏度为15.6 pg/mL。适用于科研领域对炎症机制、免疫调节相关疾病模型的深入探索，如关节炎、脓毒症等动物实验研究，可有效评估不同实验条件下IL1Ra的动态变化，为免疫调控药物开发、创伤修复研究及病理机制分析提供可靠的数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Il1rn ELISA Kit; Il-1raInterleukin-1 receptor antagonist protein ELISA Kit; IL-1RN ELISA Kit; IL-1ra ELISA Kit; IRAP ELISA Kit; IL1 inhibitor ELISA Kit
缩写：IL1RN
Uniprot No.：P25085
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays):CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1ra in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 84 | |||||
| Range % | 81-88 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 89-96 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 98-105 | ||||||
| 1:8 | Average % | 99 | |||||
| Range % | 95-104 | ||||||
回收率：
| The recovery of mouse IL-1ra spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 87 | 83-90 | |||||
| EDTA plasma (n=4) | 100 | 95-105 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 40002.5472.6012.5742.413 20001.7211.7091.7151.554 10001.0141.1021.0580.897 5000.6160.6280.6220.461 2500.3600.3710.3660.205 1250.2630.2600.2620.101 62.50.2010.1990.2000.039 00.1620.1600.161 | |||||||
货期：3-5 working days
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