Mouse Interleukin 1α,IL-1α ELISA kit

大包装询价

货号: IPD-E04621m

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠白介素1α(IL-1α)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1A含量。IL1A，即白介素-1α，是一种重要的细胞因子，参与调节免疫和炎症反应。其研究机制涉及前体蛋白的转录因子功能及成熟形式的信号传导，对细胞生长、分化和免疫应答有显著影响。试剂盒检测范围为2.35 pg/ml- 150 pg/ml，灵敏度为0.58 pg/ml。本试剂盒适用于科研领域中小鼠模型的炎症机制研究、免疫相关疾病动物模型的病理评估、药物干预效果分析等实验场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Il1aInterleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写：
IL1A
Uniprot No.：
P01582
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
2.35 pg/ml - 150 pg/ml
灵敏度：
0.58 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

回收率：

The recovery of mouse IL-1α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample TypeAverage % RecoveryRangeSerum (n=5)9390-98

标准曲线：

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-1α antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled IL-1α antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)18.59618.52918.51218.42618.54618.495SD0.0410.0460.0440.0480.0560.054CV(%)3.0103.3873.2423.5514.1213.982