Human interleukin-18 binding prorein(IL-18BP) ELISA Kit

货号: IPD-E13687h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人白介素18结合蛋白(IL-18BP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IL18BP含量。IL18BP即白介素18结合蛋白，它可与IL-18结合阻断其活性。IL-18参与机体免疫调节、炎症反应等。IL18BP作为内源性抑制剂，调控IL-18活性，有潜力用于炎症相关疾病如关节炎、感染性疾病的治疗研究。试剂盒检测范围为15.6 pg/ml-1000 pg/ml，灵敏度为3.9 pg/ml。适用于基础医学研究领域，例如探究炎症相关疾病的分子机制、评估免疫调节药物对IL - 18/IL - 18BP信号通路的影响，或作为生物标志物在心血管疾病、代谢综合征等病理模型中的动态监测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
I18BP_HUMAN ELISA Kit; IL-18BP ELISA Kit; IL18 binding protein ELISA Kit; IL18 BP ELISA Kit; IL18 BPa ELISA Kit; IL18BP ELISA Kit; IL18BPa ELISA Kit; Interleukin 18 binding protein ELISA Kit; Interleukin-18-binding protein ELISA Kit; MC51L 53L 54L homolog gene product ELISA Kit; Tadekinig alfa ELISA Kit; Tadekinig-alfa ELISA Kit
缩写：
IL18BP
Uniprot No.：
O95998
种属：
Homo sapiens (Human)
样本类型：
serum, plasma
检测范围：
15.6 pg/ml-1000 pg/ml
灵敏度：
3.9 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)128.430124.396125.740124.844128.205126.861SD0.0320.0350.0370.0500.0460.047CV(%)4.8195.4185.6757.7166.9387.154

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-18BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:10	Average %	100
	Range %	95-104
1:20	Average %	84
	Range %	80-88
1:40	Average %	98
	Range %	92-103
1:80	Average %	90
	Range %	85-97

回收率：

The recovery of human IL-18BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	93	89-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	2.412	2.624	2.518	2.412
500	1.845	1.952	1.899	1.793
250	1.181	1.124	1.153	1.047
125	0.685	0.645	0.665	0.559
62.5	0.371	0.393	0.382	0.276
31.2	0.234	0.223	0.229	0.123
15.6	0.169	0.162	0.166	0.060
0	0.107	0.105	0.106