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Human Interleukin17B (IL17B) ELISA Kit

  • 中文名: 人白介素17B (IL17B)ELISA Kit
货号: IPD-E15918h
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    人白介素17B (IL17B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的IL17B含量。IL-17B 是一种细胞因子。其它在免疫调节中起作用。研究机制上,IL-17B 与相应受体结合,激活下游信号通路,参与炎症反应、组织修复等过程,对其深入研究有助于理解免疫相关疾病发病机制,为治疗提供新方向。试剂盒检测范围为31.25 pg/ml-2000 pg/ml.,灵敏度为7.8 pg/mL。适用于基础科研中探索IL17B在免疫调控、炎症性疾病模型或肿瘤微环境中的作用机制,也可用于体外药物筛选实验中生物标志物的动态监测本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    Cytokine like protein Zcyto7 ELISA Kit; Cytokine Zcyto7 ELISA Kit; IL 17B ELISA Kit; IL 20 ELISA Kit; IL-17B ELISA Kit; IL-20 ELISA Kit; IL17B ELISA Kit; IL17B_HUMAN ELISA Kit; IL20 ELISA Kit; Interleukin 17 beta ELISA Kit; Interleukin 17B ELISA Kit; Interleukin 20 ELISA Kit; Interleukin-17B ELISA Kit; Interleukin-20 ELISA Kit; MGC138900 ELISA Kit; MGC138901 ELISA Kit; Neuronal interleukin 17 related factor ELISA Kit; Neuronal interleukin-17-related factor ELISA Kit; NIRF ELISA Kit; ZCYTO7 ELISA Kit
  • 缩写:
    IL17B
  • Uniprot No.:
    Q9UHF5
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    31.25 pg/ml-2000 pg/ml.
  • 灵敏度:
    7.8 pg/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



     Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)252.019260.431227.817252.542239.054254.114SD0.0340.0280.0320.0450.0490.042CV(%)5.0154.0355.0716.6277.5046.158







  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human IL17B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    106


    Range %

    103-109


    1:2

    Average %

    93


    Range %

    92-98


    1:4

    Average %

    92


    Range %

    86-96


    1:8

    Average %

    94


    Range %

    89-97


  • 回收率:

    The recovery of human IL17B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    103

    99-109


    EDTA plasma (n=4)

    92

    87-97









  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












    pg/ml

    OD1

    OD2

    Average

    Corrected


    2000

    2.066

    2.122

    2.094

    1.974


    1000

    1.574

    1.625

    1.600

    1.480


    500

    1.082

    1.113

    1.098

    0.978


    250

    0.643

    0.687

    0.665

    0.545


    125

    0.391

    0.406

    0.399

    0.279


    62.50

    0.256

    0.255

    0.256

    0.136


    31.25

    0.211

    0.221

    0.216

    0.096


    0

    0.119

    0.121

    0.120










    LOD

    14.8pg/ml















  • 货期:
    3-5 working days

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