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产品描述：牛免疫球蛋白M（IgM）酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IgM含量。IgM是人体内最早出现的免疫球蛋白，具有强大的杀菌和免疫调节功能。它主要通过B细胞受体（BCR）在早期免疫应答中发挥作用，并以五聚体或六聚体的形式存在。研究显示，IgM的组装和转运受J链调控，并与黏膜转运受体pIgR相互作用，参与黏膜免疫。近期研究揭示了IgM被特异性受体FcμR识别的分子机制，为深入理解其生物学功能提供了重要基础。试剂盒检测范围为0.39 μg/mL-25 μg/mL，灵敏度为0.2 μg/mL。适合用于科研领域评估牛类动物免疫机能、疫苗研发中抗体应答动态监测，或感染性疾病模型中特异性IgM水平分析，为免疫学研究提供可靠的定量工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgM
种属：Bos taurus (Bovine)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.39 μg/mL-25 μg/mL
灵敏度：0.2 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-99 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 250.0590.0560.058 12.50.0910.0990.095 6.250.1510.1520.152 3.120.3120.2920.302 1.560.6080.6600.634 0.780.8240.8010.813 0.391.1501.1921.171 01.5521.5081.530 | ||||||
货期：3-5 working days
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