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产品描述：大鼠免疫球蛋白G(IgG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgG含量。IgG即免疫球蛋白G，是人体内最为丰富的抗体类型。它在免疫防御中至关重要，能识别并结合病原体。研究机制主要围绕其结构和功能展开，解析其与抗原的结合模式，通过改造IgG结构以提升免疫治疗效果，应用于肿瘤、自身免疫病等领域。试剂盒检测范围为31.25 ng/mL-8000 ng/mL，灵敏度为31.25 ng/mL。适用于免疫学研究中评估大鼠模型体液免疫水平，例如感染性疾病、疫苗效力测试、自身免疫疾病或药物干预实验中的抗体应答监测，也可用于生物制剂研发中抗体浓度测定本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IgG
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma
检测范围：31.25 ng/mL-8000 ng/mL
灵敏度：31.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:2000 | Average % | 90 | ||||
| Range % | 86-95 | |||||
| 1:4000 | Average % | 87 | ||||
| Range % | 83-92 | |||||
| 1:8000 | Average % | 101 | ||||
| Range % | 97-106 | |||||
回收率：
| The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 88-95 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 80000.2300.2250.228 20000.4170.4070.412 5000.6430.6640.654 1251.2381.2571.248 31.251.7541.7701.762 02.0351.9882.012 | ||||||
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with rat IgG.
Standard (6 x 0.5 ml)--Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated IgG(1 x 6 ml) --Bind to the IgG, and HRP catalyzes the TMB to elicit a chromogenic reaction.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) 1 x 20 ml --Wash away unbound or free substances.
TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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