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产品描述：猪免疫球蛋白G(IgG)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IgG含量。IgG即免疫球蛋白G，是人体含量最多、最主要的抗体。它在免疫防御中起关键作用，能识别并结合抗原，激活补体系统杀伤病原体。目前在疾病治疗等方面是重要靶点，研究聚焦于通过调控其功能来开发免疫疗法。试剂盒检测范围为0.586 μg/mL-150 μg/mL，灵敏度为0.467 μg/mL。的应用场景包括：免疫机制研究、疫苗效果评估、疾病模型构建、畜牧病原体感染研究、生物制品质量控制及免疫应答动态分析等科研方向。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IgG
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.586 μg/mL-150 μg/mL
灵敏度：0.467 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:400 | Average % | 95 | ||||
| Range % | 91-107 | |||||
| 1:800 | Average % | 93 | ||||
| Range % | 86-99 | |||||
| 1:1600 | Average % | 91 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of pig IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-98 | ||||
| EDTA plasma (n=4) | 94 | 89-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 1500.2540.2510.253 37.50.5550.5170.536 9.380.7550.7210.738 2.340.9990.9810.990 0.581.1691.1131.141 01.4811.4651.473 | ||||||
货期：3-5 working days
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