首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人免疫球蛋白D(IgD)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IgD含量。IgD为免疫球蛋白D，是B细胞抗原受体的重要组成部分。其背景上在人体免疫系统中发挥关键作用。研究机制方面，它参与B细胞的活化、增殖和分化过程，在免疫应答的启动和调节中意义重大，目前对其功能机制仍在进一步深入研究。试剂盒检测范围为0.024 ng/mL-100 ng/mL，灵敏度为0.024 ng/mL。适用于免疫学基础研究、炎症机制探索或免疫相关疾病模型中IgD动态变化的定量分析；为免疫调控研究、药物效应评估或生物标志物筛选提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IgD
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.024 ng/mL-100 ng/mL
灵敏度：0.024 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 87-98 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 90-102 | |||||
| 1:400 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 86-105 | |||||
回收率：
| The recovery of human IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-96 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 1002.1202.0942.1072.005 251.6551.6371.6461.544 6.250.9951.0051.0000.898 1.560.5150.5070.5110.409 0.390.4130.4080.4110.309 0.10.2040.2100.2070.105 0.0240.1380.1320.1350.033 00.1000.1030.102 | ||||||
货期：3-5 working days
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