首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人免疫球蛋白A(IgA)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的IgA含量。IgA是一类重要的免疫球蛋白，在黏膜免疫中发挥关键作用。研究发现它能阻止病原体黏附，中和毒素等。目前围绕IgA的研究机制聚焦于其产生、调节过程，以及如何利用它开发针对感染性疾病、自身免疫病等的治疗策略。试剂盒检测范围为0.24 ng/mL-1000 ng/mL，灵敏度为0.24 ng/mL。适用于科研场景中免疫机制研究、疾病模型分析或生物样本中IgA水平的动态监测，例如肠道菌群与黏膜免疫关联性研究、慢性炎症性疾病模型的生物标志物检测等。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IgA
种属：Homo sapiens (Human)
样本类型：serum, plasma
检测范围：0.24 ng/mL-1000 ng/mL
灵敏度：0.24 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10000 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:20000 | Average % | 90 | ||||
| Range % | 86-97 | |||||
| 1:40000 | Average % | 96 | ||||
| Range % | 90-103 | |||||
| 1:80000 | Average % | 89 | ||||
| Range % | 86-93 | |||||
回收率：
| The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 89-94 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 10002.1802.2052.1932.124 2502.0932.0022.0481.979 62.51.8411.8681.8551.786 15.61.5281.5011.5151.446 3.90.7720.7530.7630.694 0.980.3960.3970.3970.328 0.240.1890.1800.1850.116 00.0690.0680.069 | ||||||
货期：3-5 working days
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