Human intercellular adhesion molecule 3,ICAM-3 ELISA Kit

中文名： 人细胞间粘附分子3(ICAM-3/CD50)ELISA Kit

货号: IPD-E07949h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人细胞间粘附分子3(ICAM-3/CD50)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的ICAM3含量。ICAM3，即细胞间粘附分子3，是一种跨膜糖蛋白，属于ICAM家族，主要功能是介导细胞间的粘附，参与免疫反应和细胞信号转导。其研究机制涉及ICAM3与LFA-1等受体的相互作用，以及其在炎症反应和肿瘤浸润中的作用。试剂盒检测范围为31.25 pg/ml- 2000 pg/ml，灵敏度为7.8 pg/ml。为研究免疫相关疾病机制、炎症模型建立、药物靶点验证等科研需求提供可靠工具，在自身免疫性疾病研究、感染免疫学实验及免疫调节剂开发等领域具有广泛应用潜力，满足实验室常规科研场景下的高效检测需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
ICAM3; Intercellular adhesion molecule 3; ICAM-3; CDw50; ICAM-R; CD antigen CD50
缩写：
ICAM3
Uniprot No.：
P32942
种属：
Homo sapiens (Human)
样本类型：
serum, plasma,tissue homogenates
检测范围：
31.25 pg/ml - 2000 pg/ml
灵敏度：
7.8 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)225.927236.470246.378218.347231.580242.642SD0.0360.0440.0310.0360.0460.029CV(%)4.2505.0523.8654.3115.3363.532

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-3/CD50 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

89-100

1:2

Average %

Range %

84-92

1:4

Average %

103

Range %

98-107

1:8

Average %

Range %

89-97

回收率：

The recovery of human ICAM-3/CD50 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	96	91-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.752	2.654	2.703	2.548
1000	1.981	2.015	1.998	1.843
500	1.407	1.386	1.397	1.242
250	0.846	0.872	0.859	0.704
125	0.529	0.508	0.519	0.364
62.5	0.318	0.321	0.320	0.165
31.25	0.235	0.229	0.232	0.077
0	0.153	0.157	0.155