首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠细胞间粘附分子1(ICAM-1/CD54)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ICAM1含量。ICAM1即细胞间黏附分子 -1，在免疫反应等生理过程中发挥重要作用。其表达异常与多种疾病相关，像炎症、肿瘤等。研究机制方面，主要聚焦于其参与细胞黏附、信号传导，可作为潜在治疗靶点，通过药物干预其活性来改善病情。试剂盒检测范围为78 ng/mL-5000 ng/mL，灵敏度为19.5 ng/mL。该产品兼容多种生物样本类型，为研究炎症机制、免疫调控、心血管疾病模型或药物干预实验提供可靠工具，例如可用于评估小鼠模型中炎症因子释放、内皮细胞激活状态或免疫细胞浸润程度。支持科研人员在体外实验中高效检测 ICAM - 1 的动态变化，为探索相关疾病的分子机制及潜在治疗靶点提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Icam1; Icam-1; Intercellular adhesion molecule 1; ICAM-1; MALA-2; MyD10; CD antigen CD54
缩写：ICAM1
Uniprot No.：P13597
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：78 ng/mL-5000 ng/mL
灵敏度：19.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:2 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 85-105 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of mouse ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-101 | ||||
| EDTA plasma (n=4) | 94 | 86-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 50002.5552.5182.5372.390 25002.0472.1052.0761.929 12501.5311.5521.5421.395 6250.8120.8030.8080.661 3120.5160.5240.5200.373 1560.3120.3210.3170.170 780.2290.2250.2270.080 00.1450.1490.147 | ||||||
货期：3-5 working days
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