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Human intercellular adhesion molecule 1,ICAM-1 ELISA Kit

  • 中文名： 人细胞间粘附分子1(ICAM-1/CD54)ELISA KIT
货号: IPD-E04574h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人细胞间粘附分子1(ICAM-1/CD54)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ICAM1含量。ICAM1即细胞间黏附分子-1，其在免疫应答、炎症反应等生理病理过程中发挥重要作用。研究机制方面，它可介导细胞间黏附与信号传递。异常表达与炎症性疾病、肿瘤转移等密切相关，因此是开发相关治疗药物的潜在靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。该产品可为炎症反应机制研究、免疫调节药物开发、心血管疾病模型建立等体外实验提供可靠数据支持，尤其适用于体外模拟炎症条件下内皮细胞活化评估、免疫细胞互作研究以及疾病相关生物标志物检测等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Antigen identified by monoclonal ELISA Kit BB2 ELISA Kit; BB 2 ELISA Kit; BB2 ELISA Kit; CD 54 ELISA Kit; CD_antigen=CD54 ELISA Kit; CD54 ELISA Kit; Cell surface glycoprotein P3.58 ELISA Kit; Human rhinovirus receptor ELISA Kit; ICAM 1 ELISA Kit; ICAM-1 ELISA Kit; ICAM1 ELISA Kit; ICAM1_HUMAN ELISA Kit; intercellular adhesion molecule 1 (CD54) ELISA Kit; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor ELISA Kit; Intercellular adhesion molecule 1 ELISA Kit; Major group rhinovirus receptor ELISA Kit; MALA 2 ELISA Kit; MALA2 ELISA Kit; MyD 10 ELISA Kit; MyD10 ELISA Kit; P3.58 ELISA Kit; Surface antigen of activated B cells ELISA Kit; Surface antigen of activated B cells, BB2 ELISA Kit
  • 缩写：
    ICAM1
  • Uniprot No.：
    P05362
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 ng/mL-50 ng/mL
  • 灵敏度：
    0.195 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:10

    Average %

    84


    Range %

    80-92


    1:20

    Average %

    94


    Range %

    91-102


    1:40

    Average %

    95


    Range %

    90-99


    1:80

    Average %

    95


    Range %

    86-99


  • 回收率：

    The recovery of human ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    96

    91-103


    EDTA plasma (n=4)

    93

    90-102


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    50

    2.438

    2.604

    2.521

    2.362


    25

    1.986

    1.897

    1.941

    1.782


    12.5

    1.408

    1.358

    1.383

    1.224


    6.25

    0.913

    0.929

    0.921

    0.762


    3.12

    0.470

    0.451

    0.460

    0.301


    1.56

    0.341

    0.353

    0.347

    0.188


    0.78

    0.259

    0.274

    0.266

    0.107


    0

    0.163

    0.155

    0.159



  • 本试剂盒所含材料：



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ICAM1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled ICAM1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料：



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期：
        3-5 working days

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