Rat High mobility group protein B1,HMGB-1 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E08224r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠高迁移率族蛋白B1(HMGB-1)酶联免疫试剂盒检测血清、血浆、组织匀浆样本中的HMGB1含量。HMGB1是一种高度保守的核蛋白，广泛存在于真核细胞中。在生理状态下，它参与维持核小体结构和调节基因转录。研究发现，细胞受损或应激时它会释放到胞外，通过与受体结合激活下游信号通路，参与炎症反应、肿瘤发生发展等多种病理过程。本试剂盒适用于实验室环境下研究炎症相关疾病的分子机制、评估实验动物模型中HMGB-1的动态变化，或用于筛选调控HMGB-1表达的药物及化合物本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Hmgb1 ELISA Kit; Hmg-1 ELISA Kit; Hmg1 ELISA Kit; High mobility group protein B1 ELISA Kit; Amphoterin ELISA Kit; Heparin-binding protein p30 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit
缩写：
HMGB1
Uniprot No.：
P63159
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
灵敏度：
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Epigenetics and Nuclear Signaling
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HMGB-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	94
	Range %	85-98
1:2	Average %	97
	Range %	91-103
1:4	Average %	102
	Range %	92-110
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
100	2.671	2.634	2.653	2.527
50	1.625	1.667	1.646	1.521
25	1.059	1.028	1.044	0.918
12.5	0.582	0.619	0.601	0.475
6.25	0.392	0.401	0.396	0.271
3.12	0.295	0.283	0.289	0.164
1.56	0.222	0.214	0.218	0.093
0	0.126	0.125	0.125