Mouse hepatocyte growth factor,HGF ELISA kit

中文名： 小鼠肝细胞生长因子(HGF)ELISA Kit

货号: IPD-E07347m

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
小鼠肝细胞生长因子(HGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测样本中的HGF含量。HGF（肝细胞生长因子）是一种由间充质细胞分泌的细胞因子，作用于多种组织和细胞类型，对癌细胞活性在肿瘤发展过程中起重要作用。HGF与c-Met受体结合后，激活酪氨酸激酶信号级联，调节细胞生长、运动和形态发生，在血管生成、肿瘤发生和组织再生中起核心作用。适用于科研领域探索肝脏疾病模型、肿瘤微环境调控、再生机制或药物干预下HGF表达水平的动态变化，为体外实验提供稳定可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Hgf ELISA Kit; Hepatocyte growth factor ELISA Kit; Hepatopoietin-A ELISA Kit; Scatter factor ELISA Kit; SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain] ELISA Kit
缩写：
HGF
Uniprot No.：
Q08048
种属：
Mus musculus (Mouse)
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)1025.6621031.3161034.6711028.4851034.6711038.297SD0.0260.0340.0410.0340.0410.048CV(%)3.8184.8365.7264.9135.7266.575

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

83-98

1:2

Average %

Range %

91-104

1:4

Average %

Range %

93-103

1:8

Average %

Range %

87-96

回收率：

The recovery of mouse HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	82-94
Platelet-poor EDTA plasma (n=4)	97	91-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.











pg/ml	OD1	OD2	Average	Corrected
8000	2.289	2.411	2.350	2.186
4000	1.830	1.700	1.765	1.601
2000	1.107	1.086	1.097	0.933
1000	0.707	0.696	0.702	0.538
500	0.448	0.437	0.443	0.279
250	0.305	0.294	0.300	0.136
125	0.246	0.250	0.248	0.084
0	0.164	0.163	0.164