Human hepatocyte growth factor,HGF ELISA kit

产品详情

中文名： 人肝细胞生长因子(HGF)ELISA kit

货号: IPD-E04573h

Price： ￥2500

48T 96T

数量：

大包装询价

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：
人肝细胞生长因子(HGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HGF含量。肝细胞生长因子（HGF）是一种多功能细胞因子，主要来源于间充质细胞，作用于上皮细胞，参与细胞生长、迁移和形态发生。HGF通过与其受体MET结合，激活下游信号通路，如PI3K-Akt和Ras-MAPK，调节多种生物学过程，包括炎症、组织修复、血管生成和肿瘤生长。HGF/MET信号通路异常与多种疾病，尤其是肿瘤的发生和发展密切相关。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于基础医学研究中的肝损伤修复模型评估、肿瘤侵袭转移机制探索，或药物研发中靶向HGF通路的干预效果验证，为细胞信号转导、再生医学及癌症生物学等领域提供可靠的定量分析支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
DFNB39 ELISA Kit; F TCF ELISA Kit; Fibroblast derived tumor cytotoxic factor ELISA Kit; Hepatocyte growth factor (hepapoietin A; scatter factor) ELISA Kit; Hepatocyte growth factor ELISA Kit; Hepatocyte growth factor beta chain ELISA Kit; Hepatocyte growth factor precursor ELISA Kit; Hepatopoietin A ELISA Kit; Hepatopoietin-A ELISA Kit; Hgf ELISA Kit; HGF_HUMAN ELISA Kit; HGFB ELISA Kit; HPTA ELISA Kit; Lung fibroblast derived mitogen ELISA Kit; OTTHUMP00000161349 ELISA Kit; OTTHUMP00000206710 ELISA Kit; OTTHUMP00000206711 ELISA Kit; OTTHUMP00000206712 ELISA Kit; OTTHUMP00000206713 ELISA Kit; OTTHUMP00000206730 ELISA Kit; Scatter factor ELISA Kit; SF ELISA Kit
缩写：
HGF
Uniprot No.：
P14210
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
15.6 pg/mL-1000 pg/mL
灵敏度：
3.9 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-98
1:2	Average %	90
	Range %	85-100
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
1000	1.873	1.953	1.913	1.723
500	1.332	1.307	1.320	1.129
250	0.839	0.903	0.871	0.680
125	0.629	0.619	0.624	0.434
62.5	0.429	0.421	0.425	0.235
31.2	0.323	0.335	0.329	0.139
15.6	0.259	0.248	0.254	0.063
0	0.189	0.193	0.191