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Human hepatitis B virus e antigen (HBeAg) ELISA Kit

  • 中文名: 人乙型肝炎病毒e抗原(HBeAg)ELISA Kit
货号: IPD-E13557h
Price: ¥3000
数量:
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产品详情

  • 产品描述:
    人乙型肝炎病毒e抗原(HBeAg)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定性检测血清、血浆样本中的HBeAg含量。HBeAg(乙型肝炎病毒e抗原)是乙型肝炎病毒的一种标志,其阳性表示病毒复制活跃,传染性强。HBeAg的研究机制主要涉及其在免疫逃逸和慢性化感染中的作用,通过抑制机体免疫应答,HBeAg帮助HBV病毒逃避免疫系统的清除,导致慢性乙型肝炎的发生。适用于科研场景中HBeAg的定性分析,如乙肝病毒感染机制研究、抗病毒药物效果评估或流行病学调查等。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 缩写:
    HBeAg
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    Request Information
  • 灵敏度:
    Request Information
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Microbiology
  • 测定原理:
    qualitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<15%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<20%




    Three samples of known concentration were tested in twenty assays to assess.



  • 标准曲线:

    Test parameter

    specification

    test result

    Positive control

    >0.8

    2.098

    Negative control

    <0.2

    0.082

    Positive rate

    10,Positive

    100%

    Negative rate

    10,Negative

    100%

  • 本试剂盒所含材料:


    • A 96-well Assay plate --The 96-well plate has been pre-coated with HBeAb.

    • Negative Control (1 x 1ml) --Eliminate false positive

    • Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.

    • HRP-conjugated HBeAb(1 x 7ml) --Bind to the HBeAg, and HRP catalyzes the TMB to elicit a chromogenic reaction.

    • Wash Buffer (20x concentrate) (1 x 30 ml) --Wash away unbound or free substances.

    • Substrate A (1 x 7 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.

  • 本试剂盒不含材料:


    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.

    • An incubator that can provide stable incubation conditions up to 37°C±5°C.

    • Centrifuge

    • Vortex

    • Squirt bottle, manifold dispenser, or automated microplate washer

    • Absorbent paper for blotting the microtiter plate

  • 货期:
    3-5 working days

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