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Intra-assay Precision (Precision within an assay): CV%<15% | |||||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||||
Inter-assay Precision (Precision between assays): CV%<20% | |||||||||
Three samples of known concentration were tested in twenty assays to assess. |
Test parameter | specification | test result | |||||
Positive control | >0.8 | 2.098 | |||||
Negative control | <0.2 | 0.082 | |||||
Positive rate | 10,Positive | 100% | |||||
Negative rate | 10,Negative | 100% |
A 96-well Assay plate --The 96-well plate has been pre-coated with HBeAb.
Negative Control (1 x 1ml) --Eliminate false positive
Positive Control (1 x 1ml) --Used to evaluate the validity, stability, and comparability of experimental results.
HRP-conjugated HBeAb(1 x 7ml) --Bind to the HBeAg, and HRP catalyzes the TMB to elicit a chromogenic reaction.
Wash Buffer (20x concentrate) (1 x 30 ml) --Wash away unbound or free substances.
Substrate A (1 x 7 ml) --Mix with substrate B and interact with HRP, eliciting a Chromogenic reaction.
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
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