Human Aspartate aminotransferase,AST ELISA Kit

中文名： 人天门冬氨酸氨基转移酶(AST)ELISA Kit

货号: IPD-E09603h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人天门冬氨酸氨基转移酶(AST)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GOT1含量。GOT1即天冬氨酸转氨酶 1，在细胞代谢尤其是氨基酸代谢、三羧酸循环中起关键作用。研究发现其在肿瘤发生发展中机制复杂，它参与维持肿瘤细胞增殖所需代谢物供应，还与肿瘤细胞的氧化还原稳态相关，是潜在的肿瘤治疗靶点。试剂盒检测范围为0.312 mU/ml- 20 mU/ml，灵敏度为0.078 mU/ml。在基础医学研究中广泛用于评估肝细胞功能状态、药物毒性作用或心血管疾病模型的病理进程；特别适用于体外实验中监测细胞或组织样本的 AST 释放量，例如在肝损伤动物模型研究中评估组织匀浆酶活性变化，或通过血清/血浆样本分析代谢性疾病进程中细胞膜的完整性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
AATC_HUMAN ELISA Kit; Aspartate aminotransferase 1 ELISA Kit; Aspartate aminotransferase ELISA Kit; Aspartate aminotransferase cytoplasmic ELISA Kit; Aspartate aminotransferase cytosolic ELISA Kit; ASTQTL1 ELISA Kit; cAspAT ELISA Kit; cCAT ELISA Kit; cysteine aminotransferase; cytoplasmic ELISA Kit; cysteine transaminase; cytoplasmic ELISA Kit; cytoplasmic ELISA Kit; ec 2.6.1.1 ELISA Kit; GIG 18 ELISA Kit; GIG18 ELISA Kit; Glutamate oxaloacetate transaminase 1 ELISA Kit; Glutamate oxaloacetate transaminase soluble ELISA Kit; Glutamic oxaloacetic transaminase 1 soluble ELISA Kit; GOT 1 ELISA Kit; GOT1 ELISA Kit; Growth inhibiting protein 18 ELISA Kit; SGOT ELISA Kit; Transaminase A ELISA Kit
缩写：
GOT1
Uniprot No.：
P17174
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.312 mU/ml - 20 mU/ml
灵敏度：
0.078 mU/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(mU/ml)	2.283	2.469	2.072	2.060	2.283	2.346
SD	0.044	0.045	0.038	0.038	0.044	0.047
CV(%)	6.607	6.475	6.022	6.041	6.607	6.953

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-88

1:2

Average %

Range %

88-94

1:4

Average %

Range %

90-99

1:8

Average %

103

Range %

97-110

回收率：

The recovery of human AST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	90	85-94
EDTA plasma(n=4)	95	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.





mU/ml	OD1	OD2	Average	Corrected
20	2.368	2.401	2.384	2.251
10	1.439	1.442	1.440	1.307
5	1.015	1.006	1.011	0.878
2.5	0.665	0.642	0.654	0.521
1.25	0.441	0.435	0.438	0.305
0.625	0.347	0.351	0.349	0.216
0.312	0.201	0.204	0.203	0.070
0	0.135	0.132	0.133