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Mouse gonadotropin-releasing hormone,GnRH ELISA Kit

  • 中文名： 小鼠促性腺激素释放激素(GnRH)ELISA Kit
货号: IPD-E08152m
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    小鼠促性腺激素释放激素(GnRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GNRH1含量。GNRH1，即促性腺激素释放激素1，是一种由下丘脑分泌的多肽激素，主要功能是刺激垂体释放卵泡刺激素（FSH）和黄体生成素（LH），从而调控生殖系统的发育和功能。其研究机制涉及GnRH1与垂体细胞膜上受体的结合，进而启动复杂的信号通路，调节性激素的分泌。试剂盒检测范围为7.8 pg/ml- 500 pg/ml，灵敏度为1.95 pg/ml。适用于实验室环境下探究生殖生理调控、卵巢/睾丸功能相关疾病模型的激素变化、药物干预对下丘脑 - 垂体 - 性腺轴的影响等科研场景，为神经内分泌研究、生殖医学基础实验及药效评估提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Gnrh1 ELISA Kit; GnrhProgonadoliberin-1 ELISA Kit; Progonadoliberin I) [Cleaved into: Gonadoliberin-1 ELISA Kit; Gonadoliberin I ELISA Kit; Gonadotropin-releasing hormone I ELISA Kit; GnRH-I ELISA Kit; Luliberin I ELISA Kit; Luteinizing hormone-releasing hormone I ELISA Kit; LH-RH I); Prolactin release-inhibiting factor 1 ELISA Kit; Prolactin release-inhibiting factor I)] ELISA Kit
  • 缩写：
    GNRH1
  • Uniprot No.：
    P13562
  • 种属：
    Mus musculus (Mouse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 pg/ml - 500 pg/ml
  • 灵敏度：
    1.95 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of mouse GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    94


    Range %

    86-99


    1:2

    Average %

    95


    Range %

    91-105


    1:4

    Average %

    92


    Range %

    88-97


    1:8

    Average %

    93


    Range %

    86-98


  • 回收率：

    The recovery of mouse GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    94

    89-98


    EDTA plasma (n=4)

    92

    85-98


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    500

    1.961

    1.834

    1.897

    1.717


    250

    1.272

    1.267

    1.270

    1.089


    125

    0.742

    0.728

    0.735

    0.554


    62.5

    0.456

    0.429

    0.442

    0.261


    31.2

    0.301

    0.315

    0.308

    0.127


    15.6

    0.259

    0.253

    0.256

    0.075


    7.8

    0.205

    0.197

    0.201

    0.020


    0

    0.177

    0.185

    0.181




  • 货期：
    3-5 working days

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