Human growth differentiation factor 2,GDF2 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E11769h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人生长分化因子2(GDF2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的GDF2含量。GDF2 即生长分化因子 2，在多种生物学过程中发挥关键作用。研究发现它参与血管生成、骨发育等机制。其通过与特定受体结合激活信号通路来调控细胞行为，在疾病发生发展中也有潜在影响，是药物研发等领域的潜在靶点。试剂盒检测范围为3.9 pg/mL-250 pg/mL，灵敏度为0.98 pg/mL。该产品适用于基础科研中探究心血管疾病模型、代谢紊乱或组织修复过程中GDF2的动态表达，也可用于体外细胞培养上清或基因编辑动物模型的蛋白水平验证，为分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
GDF2 ELISA Kit; BMP9Growth/differentiation factor 2 ELISA Kit; GDF-2 ELISA Kit; Bone morphogenetic protein 9 ELISA Kit; BMP-9 ELISA Kit
缩写：
GDF2
Uniprot No.：
Q9UK05
种属：
Homo sapiens (Human)
样本类型：
serum, plasma,tissue homogenates
检测范围：
3.9 pg/mL-250 pg/mL
灵敏度：
0.98 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Others
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GDF2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	85-93
1:2	Average %	101
	Range %	96-105
1:4	Average %	94
	Range %	88-99
1:8	Average %	104
	Range %	97-108

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
250	2.112	2.214	2.163	2.065
125	1.241	1.341	1.291	1.193
62.5	0.786	0.739	0.763	0.665
31.25	0.438	0.448	0.443	0.345
15.6	0.287	0.297	0.292	0.194
7.8	0.154	0.169	0.162	0.064
3.9	0.131	0.137	0.134	0.036
0	0.097	0.099	0.098