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Human growth differentiation factor 15(GDF15)ELISA Kit

  • 中文名: 人生长分化因子15(GDF15)ELISA Kit
货号: IPD-E12009h
Price: ¥2500
数量:
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产品详情

  • 产品描述:人生长分化因子15(GDF15)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、尿液样本中的GDF15含量。GDF15是一种生长分化因子。其它在多种生理病理过程中发挥作用。研究机制上,GDF15可通过结合特定受体调控细胞生长、分化等活动,与代谢、肿瘤、心血管疾病等的发生发展存在关联,在疾病诊断和治疗上有潜在价值。试剂盒检测范围为7.8 pg/mL-500 pg/mL,灵敏度为1.95 pg/mL。该产品适用于基础科研中探究GDF15在代谢调控、肿瘤进展或慢性炎症等领域的分子机制,也可用于体外模型或动物实验中生物标志物的定量分析,为疾病相关分子通路研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:GDF 15 ELISA Kit; GDF-15 ELISA Kit; Gdf15 ELISA Kit; GDF15_HUMAN ELISA Kit; Growth differentiation factor 15 ELISA Kit; Growth/differentiation factor 15 ELISA Kit; Macrophage inhibitory cytokine 1 ELISA Kit; MIC 1 ELISA Kit; Mic-1 ELISA Kit; MIC1 ELISA Kit; NAG 1 ELISA Kit; NAG-1 ELISA Kit; NAG1 ELISA Kit; NRG 1 ELISA Kit; NRG-1 ELISA Kit; NRG1 ELISA Kit; NSAID (nonsteroidal anti inflammatory drug) activated protein 1 ELISA Kit; NSAID ELISA Kit; NSAID regulated protein 1 ELISA Kit; NSAID-activated gene 1 protein ELISA Kit; NSAID-regulated gene 1 protein ELISA Kit; PDF ELISA Kit; PLAB ELISA Kit; Placental bone morphogenetic protein ELISA Kit; Placental bone morphogenic protein ELISA Kit; Placental TGF beta ELISA Kit; Placental TGF-beta ELISA Kit; Prostate differentiation factor ELISA Kit; PTGF beta ELISA Kit; PTGFB ELISA Kit

  • 缩写:GDF15

  • Uniprot No.:Q99988

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates, urine

  • 检测范围:7.8 pg/mL-500 pg/mL

  • 灵敏度:1.95 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Signal Transduction

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human GDF15 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:1Average %89
    Range %80-91
    1:2Average %98
    Range %91-104
    1:4Average %97
    Range %90-105
    1:8Average %95
    Range %91-100
  • 回收率:

    The recovery of human GDF15 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9286-99
    EDTA plasma (n=4)9791-105














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected 5001.7561.7371.7471.601 2501.1341.1421.1380.992 1250.7020.7190.7110.565 62.50.4650.4500.4580.312 31.20.3370.3490.3430.197 15.60.2660.2720.2690.123 7.80.1920.2030.1980.052 00.1450.1470.146  





  • 本试剂盒所含材料:



    • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GDF15 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.

    • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.

    • One vial Biotin-labeled GDF15 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.

    • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.

    • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.

    • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.

    • 本试剂盒不含材料:



      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.

      • An incubator can provide stable incubation conditions up to 37°C±5°C.

      • Centrifuge

      • Vortex

      • Squirt bottle, manifold dispenser, or automated microplate washer

      • Absorbent paper for blotting the microtiter plate


      • 货期:3-5 working days

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