Human Galectin 7(Gal-7) ELISA Kit

中文名： 人半乳糖凝集素-7(Gal-7)ELISA Kit

货号: IPD-E13454h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人半乳糖凝集素-7（Gal-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Gal-7含量。Gal-7，也称为LGALS7，是一种参与细胞-细胞和细胞-基质相互作用的蛋白质，可能对正常生长控制至关重要。它作为促凋亡因子，在细胞内发挥作用，通过JNK激活和细胞色素c释放的上游促进细胞凋亡途径。研究显示，Gal-7在多种鳞状上皮癌中异常高表达，其靶向价值在癌症诊断和治疗中具有潜在应用。试剂盒检测范围为78 pg/ml-5000 pg/ml，灵敏度为19.5 pg/ml。该产品适用于科研领域对Gal-7蛋白表达水平的精准分析，例如探究炎症性疾病、皮肤病理模型或肿瘤微环境中Gal-7的动态变化规律，也可用于体外培养细胞或动物实验样本的分子机制研究，为探索Gal-7在免疫调控、细胞间信号传导等生物学过程中的功能提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Gal-7 ELISA Kit; GAL7 ELISA Kit; Galectin 7B ELISA Kit; Galectin-7 ELISA Kit; HKL-14 ELISA Kit; Human keratinocyte lectin 14 ELISA Kit; Keratinocyte lectin 14 ELISA Kit; Lectin galactoside binding soluble 7 ELISA Kit; Lectin; galactoside binding; soluble; 7B ELISA Kit; LEG7_HUMAN ELISA Kit; LGALS7 ELISA Kit; LGALS7A ELISA Kit; LGALS7B ELISA Kit; P53 induced protein 1 ELISA Kit; p53-induced gene 1 protein ELISA Kit; Pi7 ELISA Kit; PIG1 ELISA Kit; TP53I1 ELISA Kit
缩写：
Gal-7
Uniprot No.：
P47929
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/ml-5000 pg/ml
灵敏度：
19.5 pg/ml
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)644.292616.477630.349622.774649.379631.614SD0.0300.0350.0350.0410.0480.043CV(%)4.2495.1175.0365.9516.7616.178

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Gal-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	91
	Range %	88-96
1:2	Average %	95
	Range %	90-102
1:4	Average %	97
	Range %	94-104
1:8	Average %	101
	Range %	95-105

回收率：

The recovery of human Gal-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-96
EDTA plasma (n=4)	98	95-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.












pg/ml	OD1	OD2	Average	Corrected
5000	2.819	2.910	2.865	2.739
2500	1.857	1.830	1.844	1.718
1250	1.140	1.057	1.099	0.973
625	0.750	0.729	0.740	0.614
312	0.422	0.422	0.422	0.296
156	0.291	0.311	0.301	0.175
78	0.183	0.177	0.180	0.054
0	0.123	0.129	0.126