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Human acidic fibroblast growth factor,aFGF/FGF-1 ELISA Kit

  • 中文名： 人酸性成纤维细胞生长因子(aFGF/FGF-1)ELISA Kit
货号: IPD-E04546h
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    人酸性成纤维细胞生长因子(aFGF/FGF-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF1含量。FGF1 即成纤维细胞生长因子 1，是一种多功能细胞因子。其在细胞增殖、分化及组织修复等生理过程起重要作用。研究机制方面，它主要通过与特定受体结合激活下游信号通路，影响基因表达，在肿瘤、糖尿病等疾病研究中受关注。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，灵敏度为3.9 pg/mL。适用于体外实验中对aFGF表达水平的动态监测，例如研究其在组织再生、炎症调控或疾病模型（如缺血再灌注损伤、肿瘤微环境）中的生物学功能。科研人员可通过该产品深入探索aFGF在药物干预、基因表达调控或病理状态下的浓度变化，为揭示其分子机制及与其他信号通路的相互作用提供可靠数据支持，助力基础研究与转化医学领域的相关探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Acidic fibroblast growth factor ELISA Kit; aFGF ELISA Kit; Beta endothelial cell growth factor ELISA Kit; Beta-endothelial cell growth factor ELISA Kit; ECGF ELISA Kit; ECGF beta ELISA Kit; ECGF-beta ELISA Kit; ECGFA ELISA Kit; ECGFB ELISA Kit; Endothelial Cell Growth Factor alpha ELISA Kit; Endothelial Cell Growth Factor beta ELISA Kit; FGF 1 ELISA Kit; FGF alpha ELISA Kit; Fgf1 ELISA Kit; FGF1_HUMAN ELISA Kit; FGFA ELISA Kit; Fibroblast Growth Factor 1 Acidic ELISA Kit; Fibroblast growth factor 1 ELISA Kit; GLIO703 ELISA Kit; HBGF 1 ELISA Kit; HBGF-1 ELISA Kit; HBGF1 ELISA Kit; Heparin binding growth factor 1 ELISA Kit; Heparin binding growth factor 1 precursor ELISA Kit; Heparin-binding growth factor 1 ELISA Kit
  • 缩写：
    FGF1
  • Uniprot No.：
    P05230
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of human aFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    91


    Range %

    86-95


    1:2

    Average %

    102


    Range %

    97-107


    1:4

    Average %

    91


    Range %

    85-97


    1:8

    Average %

    97


    Range %

    91-103


  • 回收率：

    The recovery of human aFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    95

    89-98


    EDTA plasma (n=4)

    97

    90-100


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.471

    2.498

    2.485

    2.319


    500

    1.827

    1.868

    1.848

    1.682


    250

    1.369

    1.366

    1.368

    1.202


    125

    0.898

    0.861

    0.880

    0.714


    62.5

    0.474

    0.461

    0.468

    0.302


    31.2

    0.334

    0.352

    0.343

    0.177


    15.6

    0.234

    0.236

    0.235

    0.069


    0

    0.164

    0.168

    0.166




  • 货期：
    3-5 working days

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