Human Fetuin-B(FETUB) ELISA kit

货号: IPD-EL008598HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人胎球蛋白B(FETUB)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FETUB含量。试剂盒检测范围为0.137 ng/ml-100 ng/ml，灵敏度为0.137 ng/ml。适用于科研领域对血清、血浆及组织匀浆样本中FETUB蛋白含量的精准分析；为研究者探索FETUB在代谢调控、肝脏病理机制中的生物学功能提供可靠工具；用于相关疾病模型或干预实验中生物标志物的动态监测；适用于细胞实验、动物模型研究及人类样本分析等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
16G2 ELISA Kit; 2310011O17Rik ELISA Kit; AI255764 ELISA Kit; D17980 ELISA Kit; Fet ELISA Kit; Fetub ELISA Kit; FETUB_HUMAN ELISA Kit; Fetuin beta ELISA Kit; Fetuin-B ELISA Kit; Fetuin-like protein ELISA Kit; Fetuin-like protein IRL685 ELISA Kit; Gugu ELISA Kit; IRL685 ELISA Kit; Pp63 ELISA Kit
缩写：
FETUB
Uniprot No.：
Q9UGM5
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
0.137 ng/ml-100 ng/ml.
灵敏度：
0.137 ng/ml.
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cardiovascular
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(ng/ml)12.72013.07912.87412.64312.82312.925SD0.0230.0270.0250.0360.0330.031CV(%)3.6224.1603.9005.6965.1644.821

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human Fetuin B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:10	Average %	106
	Range %	102-108
1:20	Average %	88
	Range %	84-92
1:40	Average %	92
	Range %	85-96
1:80	Average %	87
	Range %	82-83

回收率：

The recovery of human Fetuin B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	104	98-108
EDTA plasma (n=4)	98	94-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
100	2.775	2.859	2.817	2.665
33.33	1.951	1.937	1.944	1.792
11.11	1.090	1.062	1.076	0.924
3.7	0.642	0.691	0.666	0.514
1.23	0.385	0.364	0.375	0.223
0.41	0.283	0.285	0.284	0.132
0.137	0.218	0.235	0.226	0.074
0	0.151	0.153	0.152