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产品描述：人凋亡相关因子配体(FASL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的FASLG含量。FASLG是FAS配体。其背景上，它在免疫调节、细胞凋亡等生理过程中意义重大。研究机制上，FASLG与FAS受体结合后，能激活细胞内凋亡信号通路，诱导靶细胞凋亡，在肿瘤免疫、自身免疫病等研究领域备受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.81 pg/mL。科研人员可通过探究FASL在细胞程序性死亡、免疫逃逸机制中的调控作用，或用于肿瘤免疫治疗、炎症性疾病模型等研究中生物标志物的动态监测，为揭示FAS/FASL信号通路在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALPS1B ELISA Kit; Apoptosis (APO 1) antigen ligand 1 ELISA Kit; Apoptosis antigen ligand 1 ELISA Kit; Apoptosis antigen ligand ELISA Kit; APT1LG1 ELISA Kit; APTL ELISA Kit; CD178 ELISA Kit; CD178 antigen ELISA Kit; CD95 ligand ELISA Kit; CD95-L ELISA Kit; CD95L ELISA Kit; CD95L protein ELISA Kit; Fas antigen ligand ELISA Kit; Fas L ELISA Kit; Fas ligand (TNF superfamily member 6) ELISA Kit; Fas ligand ELISA Kit; FASL ELISA Kit; Fasl Fas ligand (TNF superfamily member 6) ELISA Kit; FASLG ELISA Kit; Generalized lymphoproliferative disease ELISA Kit; Gld ELISA Kit; soluble form ELISA Kit; TNFL6_HUMAN ELISA Kit; TNFSF6 ELISA Kit; Tumor necrosis factor (ligand) superfamily member 6 ELISA Kit; Tumor necrosis factor ligand superfamily member 6 ELISA Kit
缩写：FASLG
Uniprot No.：P48023
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human FASL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 89-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 93-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-94 | |||||
回收率：
| The recovery of human FASL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.4722.6242.5482.423 10001.5681.4671.5181.393 5000.8460.8660.8560.731 2500.4880.4740.4810.356 1250.3210.3320.3270.202 62.50.2550.2670.2610.136 31.250.1920.1980.1950.070 00.1240.1260.125 | ||||||
货期：3-5 working days
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