Human Factor-related Apoptosis,FAS ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E04542h

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人凋亡相关因子(FAS/CD95)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FAS含量。FAS是重要靶点。它属肿瘤坏死因子受体超家族，在细胞凋亡调控等生理病理过程中起关键作用。研究机制上，FAS与其配体FASL结合，启动凋亡信号通路，相关研究对癌症、自身免疫病等治疗策略开发意义重大。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，灵敏度为7.8 pg/mL。适用于体外研究细胞凋亡调控机制、评估免疫相关疾病模型中FAS/CD95的表达变化，或作为基础研究工具用于肿瘤生物学、药物靶点筛选等领域的蛋白定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
FAS; APT1; FAS1; TNFRSF6; Tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; CD antigen CD95
缩写：
FAS
Uniprot No.：
P25445
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
31.25 pg/mL-2000 pg/mL
灵敏度：
7.8 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cell Biology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	104
	Range %	98-111
1:4	Average %	90
	Range %	85-94
1:8	Average %	84
	Range %	80-88

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2000	2.161	2.150	2.156	1.961
1000	1.457	1.432	1.445	1.250
500	1.052	0.976	1.014	0.819
250	0.672	0.697	0.685	0.490
125	0.416	0.392	0.404	0.209
32.5	0.312	0.327	0.320	0.125
31.25	0.256	0.268	0.262	0.067
0	0.201	0.189	0.195