Human Seprase(FAP) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL008424HU

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
人成纤维细胞活化蛋白α(FAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆,组织匀浆样本中的FAP含量。FAP（成纤维细胞活化蛋白）是一种跨膜丝氨酸蛋白酶，在多种实体肿瘤的癌相关成纤维细胞中高表达，而在正常组织中几乎不表达。研究其机制主要围绕利用它的特异性，开发针对性药物，如抗体偶联药物等，以精准打击肿瘤微环境，为肿瘤治疗带来新策略。试剂盒检测范围为0.312 ng/mL-20 ng/mL，灵敏度为0.078 ng/mL。适用于科研领域对肿瘤微环境、纤维化疾病机制的研究，或通过检测生物样本中FAPα表达水平探索其与疾病进展的关联性本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
170 kDa melanoma membrane bound gelatinase ELISA Kit; 170 kDa melanoma membrane-bound gelatinase ELISA Kit; DPPIV ELISA Kit; FAP ELISA Kit; FAPA ELISA Kit; Fibroblast activation protein alpha ELISA Kit; Integral membrane serine protease ELISA Kit; SEPR_HUMAN ELISA Kit; Seprase ELISA Kit
缩写：
FAP
Uniprot No.：
Q12884
种属：
Homo sapiens (Human)
样本类型：
serum, plasma,tissue homogenates
检测范围：
0.312 ng/mL-20 ng/mL
灵敏度：
0.078 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Cancer
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human FAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:2	Average %	99
	Range %	95-103
1:4	Average %	87
	Range %	83-93
1:8	Average %	95
	Range %	89-99
1:16	Average %	88
	Range %	82-94

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
20	2.864	2.655	2.760	2.665
10	2.308	2.214	2.261	2.166
5	1.637	1.533	1.585	1.490
2.5	0.827	0.805	0.816	0.721
1.25	0.476	0.467	0.472	0.377
0.625	0.289	0.272	0.281	0.186
0.312	0.184	0.178	0.181	0.086
0	0.096	0.094	0.095