Human heart fatty acid binding protein,h-FABP ELISA Kit

中文名： 人心型脂肪酸结合蛋白(h-FABP)ELISA Kit

货号: IPD-E09185h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人心型脂肪酸结合蛋白(h-FABP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FABP3含量。FABP3（脂肪酸结合蛋白3）是脂肪酸结合蛋白家族的一员，参与长链脂肪酸的摄取、细胞内代谢和运输。研究显示，FABP3在调节细胞生长、增殖及骨代谢中发挥重要作用，其异常表达与骨质疏松症等疾病相关。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。支持科研人员高效开展心肌损伤机制研究、心血管药物作用评估或疾病模型中h - FABP的动态监测，尤其适合在细胞实验、动物模型或离体组织分析中探索心肌代谢与病理生理关联性。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
422 protein ELISA Kit; Cardiac FABP ELISA Kit; Cardiac Fatty Acid Binding Protein ELISA Kit; FABP 11 ELISA Kit; FABP 3 ELISA Kit; FABP11 ELISA Kit; FABP3 ELISA Kit; FABPH_HUMAN ELISA Kit; Fatty acid binding protein 11 ELISA Kit; Fatty acid binding protein 3 ELISA Kit; Fatty acid binding protein 3 muscle and heart ELISA Kit; Fatty acid binding protein 3 muscle and heart mammary derived growth inhibitor ELISA Kit; Fatty acid binding protein 3 muscle ELISA Kit; Fatty acid binding protein 3; muscle and heart (mammary derived growth inhibitor) ELISA Kit; Fatty acid binding protein 3; muscle ELISA Kit; Fatty acid binding protein heart ELISA Kit; Fatty acid binding protein; heart ELISA Kit; Fatty acid binding protein; muscle and heart ELISA Kit; Fatty acid binding protein; skeletal muscle ELISA Kit; Fatty acid-binding protein 3 ELISA Kit; Fatty acid-binding protein ELISA Kit; H FABP ELISA Kit; H-FABP ELISA Kit; heart ELISA Kit; Heart type fatty acid binding protein ELISA Kit; Heart-type fatty acid-binding protein ELISA Kit; M FABP ELISA Kit; M-FABP ELISA Kit; Mammary derived growth inhibitor ELISA Kit; Mammary-derived growth inhibitor ELISA Kit; MDGI ELISA Kit; Muscle fatty acid binding protein ELISA Kit; Muscle fatty acid-binding protein ELISA Kit; Mylein protein P2 homolog ELISA Kit; O FABP ELISA Kit; OTTHUMP00000003898 ELISA Kit; P2 adopocyte protein ELISA Kit
缩写：
FABP3
Uniprot No.：
P05413
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	644.672	654.959	663.899	676.001	616.120	630.243
SD	0.044	0.045	0.048	0.048	0.054	0.057
CV(%)	4.955	5.028	5.327	5.281	6.221	6.492

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

80-90

1:2

Average %

Range %

89-100

1:4

Average %

Range %

91-107

1:8

Average %

Range %

82-98

回收率：

The recovery of human FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range%
Serum (n=5)	93	85-101
EDTA plasma(n=4)	95	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.




pg/ml	OD1	OD2	Average	Corrected
5000	2.215	2.292	2.254	2.150
2500	1.623	1.576	1.600	1.496
1250	1.216	1.184	1.200	1.096
625	0.851	0.839	0.845	0.741
312	0.518	0.504	0.511	0.407
156	0.291	0.279	0.285	0.181
78	0.127	0.119	0.123	0.019
0	0.108	0.100	0.104