首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人神经特异性烯醇化酶(NSE)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ENO2含量。ENO2又名烯醇化酶2，是一种糖酵解酶。它在神经元中高度表达，在神经发育、维持神经功能等方面有重要作用。研究发现，它与神经母细胞瘤等多种肿瘤的发生发展相关，可能通过调节糖代谢和肿瘤微环境等机制影响肿瘤细胞的生长与存活。试剂盒检测范围为0.78 ng/mL-50 ng/mL，灵敏度为0.195 ng/mL。适用于神经疾病机制研究、肿瘤标志物筛选、药物作用评估等科研领域，尤其可用于体外模型中神经损伤修复、肿瘤微环境调控等实验的NSE动态监测，为神经生物学、肿瘤学及转化医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2 phospho D glycerate hydrolyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Eno 2 ELISA Kit; ENO2 ELISA Kit; ENOG ELISA Kit; ENOG_HUMAN ELISA Kit; Enolase 2 (gamma; neuronal) ELISA Kit; Enolase 2 ELISA Kit; Enolase 2 gamma neuronal ELISA Kit; Enolase2 ELISA Kit; Epididymis secretory protein Li 279 ELISA Kit; Gamma enolase ELISA Kit; Gamma-enolase ELISA Kit; HEL S 279 ELISA Kit; Neural enolase ELISA Kit; Neuron specific enolase ELISA Kit; Neuron specific gamma enolase ELISA Kit; Neuron-specific enolase ELISA Kit; neuronal enriched enolase ELISA Kit; Neurone specific enolase ELISA Kit; NSE ELISA Kit
缩写：ENO2
Uniprot No.：P09104
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NSE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 84-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 95-104 | |||||
| 1:8 | Average % | 102 | ||||
| Range % | 96-108 | |||||
回收率：
| The recovery of human NSE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 88-96 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected 502.3412.3322.3372.240 251.9811.9781.9801.883 12.51.5491.5321.5411.444 6.251.0140.9931.0040.907 3.120.6580.6340.6460.549 1.560.3960.3820.3890.292 0.780.2920.2880.2900.193 00.0980.0950.097 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human NSE antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled NSE antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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