首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人核心蛋白聚糖(DCN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DCN含量。DCN（Decorin）是一种细胞外基质的核心蛋白聚糖，参与细胞信号传导、发育和癌症等生理和病理过程的调控。其研究机制涉及Dcn基因的序列特征、蛋白结构和性质，以及其在猪卵巢颗粒细胞凋亡中的作用。DCN在哺乳动物进化过程中较为保守，是猪卵巢颗粒细胞的促凋亡因子。试剂盒检测范围为3.9 pg/mL-250 pg/mL，灵敏度为0.97 pg/mL。适用于体外科研场景中DCN表达水平分析，如组织工程中细胞外基质动态研究、肿瘤微环境相关机制探索或纤维化疾病模型中基质重构评估，为细胞生物学、再生医学及病理机制研究提供定量工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Bone proteoglycan II ELISA Kit; CSCD ELISA Kit; DCN ELISA Kit; DCN protein ELISA Kit; Decorin ELISA Kit; Decorin proteoglycan ELISA Kit; Dermatan sulphate proteoglycans II ELISA Kit; DKFZp686J19238 ELISA Kit; DSPG 2 ELISA Kit; DSPG2 ELISA Kit; PG 40 ELISA Kit; PG II ELISA Kit; PG S2 ELISA Kit; PG-S2 ELISA Kit; PG40 ELISA Kit; PGII ELISA Kit; PGS 2 ELISA Kit; PGS2 ELISA Kit; PGS2_HUMAN ELISA Kit; Proteoglycan core protein ELISA Kit; SLRR1B ELISA Kit; Small leucine rich protein 1B ELISA Kit
缩写：DCN
Uniprot No.：P07585
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates.
检测范围：3.9 pg/mL-250 pg/mL
灵敏度：0.97 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DCN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||
| Sample | Serum(n=4) | |||
| 1:10 | Average % | 95 | ||
| Range % | 91-99 | |||
| 1:20 | Average % | 103 | ||
| Range % | 98-106 | |||
| 1:40 | Average % | 110 | ||
| Range % | 106-114 | |||
| 1:80 | Average % | 92 | ||
| Range % | 89-97 |
回收率：
| The recovery of human DCN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 84 | 81-89 | |||||
| EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 2501.9441.9681.9561.815 1251.2841.2321.2581.117 62.50.7500.7710.7610.620 31.20.5120.5020.5070.366 15.60.3740.3680.3710.230 7.80.2580.2620.2600.119 3.90.1940.1990.1970.056 00.1400.1420.141 | |||||||
货期：3-5 working days
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