Human granulocyte chemotactic protein-2,GCP-2 ELISA Kit

中文名： 人粒细胞趋化蛋白-2(GCP-2/CXCL6)ELISA Kit

货号: IPD-E09990h

Price： ￥2500

48T 96T

数量：

大包装询价

产品详情

产品描述：
人粒细胞趋化蛋白-2(GCP-2/CXCL6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL6含量。CXCL6，也称为粒细胞趋化蛋白-2（GCP-2），是CXC趋化因子家族的一员，具有中性粒细胞活化和血管生成活性，对革兰氏阴性菌和革兰氏阳性菌具有抗菌作用。其研究机制涉及通过CXCR1和CXCR2受体启动趋化性，激活内皮细胞引起有丝分裂反应，导致血管生成。CXCL6在炎症和癌症中扮演重要角色，是潜在的治疗靶点。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于体外实验中对炎症机制研究、药物干预效果评估或疾病模型中趋化因子动态变化的定量分析，例如在细胞因子网络研究、免疫调节实验或炎症相关动物模型的组织样本检测中本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
C X C motif chemokine 6 ELISA Kit; Chemokine (C X C motif) ligand 6 ELISA Kit; Chemokine alpha 3 ELISA Kit; CKA 3 ELISA Kit; CKA-3 ELISA Kit; CKA3 ELISA Kit; CXCL 6 ELISA Kit; CXCL6 ELISA Kit; CXCL6_HUMAN ELISA Kit; GCP 2 ELISA Kit; GCP-2 ELISA Kit; Granulocyte chemotactic protein 2 ELISA Kit; N-processed variant 3 ELISA Kit; SCYB6 ELISA Kit; Small inducible cytokine B6 ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member 6 ELISA Kit; Small inducible cytokine subfamily B (Cys X Cys) member b ELISA Kit; Small-inducible cytokine B6 ELISA Kit
缩写：
CXCL6
Uniprot No.：
P80162
种属：
Homo sapiens (Human)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

　Intra-Assay PrecisionInter-Assay PrecisionSample123123n202020202020Mean(pg/ml)624.092617.035632.238637.984631.667633.154SD0.0320.0400.0490.0510.0550.060CV(%)3.6835.3986.2746.5446.2946.762

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GCP-2/CXCL6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

81-88

1:2

Average %

Range %

89-96

1:4

Average %

102

Range %

98-105

1:8

Average %

Range %

95-104

回收率：

The recovery of human GCP-2/CXCL6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-90
EDTA plasma (n=4)	100	95-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.



pg/ml	OD1	OD2	Average	Corrected
5000	2.750	2.842	2.796	2.658
2500	1.947	2.055	2.001	1.863
1250	1.201	1.280	1.241	1.103
625	0.763	0.798	0.781	0.643
312	0.461	0.433	0.447	0.309
156	0.286	0.293	0.290	0.152
78	0.233	0.216	0.225	0.087
0	0.170	0.180	0.175