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产品描述:人上皮中性粒细胞活化肽78(ENA-78/CXCL5)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的CXCL5含量。CXCL5是一种重要靶点。它是趋化因子,在多种生理和病理过程中发挥作用。研究机制方面,其可与受体结合,调节细胞趋化活动,参与炎症反应、肿瘤生长与转移等过程,对它的深入研究有望为相关疾病治疗提供新方向。试剂盒检测范围为0.625 ng/mL-40 ng/mL,灵敏度为0.567 ng/mL。适用于科研领域对CXCL5表达水平的定量分析,例如探究肿瘤发生发展、感染性疾病进程或创伤后炎症反应等机制研究,为体外实验提供可靠的蛋白浓度数据支持本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:AMCFII ELISA Kit; C-X-C motif chemokine 5 ELISA Kit; C-X-C motif chemokine ligand 5 ELISA Kit; Chemokine (C X C motif) ligand 5 ELISA Kit; chemokine (C-X-C motif) ligand 5 ELISA Kit; Cxcl5 ELISA Kit; CXCL5_HUMAN ELISA Kit; ENA 78 ELISA Kit; ENA-78 (8-78) ELISA Kit; ENA-78(1-78) ELISA Kit; ENA-78(9-78) ELISA Kit; ENA78 ELISA Kit; Epithelial derived neutrophil activating protein 78 ELISA Kit; Epithelial-derived neutrophil-activating protein 78 ELISA Kit; Lipopolysaccharide-induced CXC chemokine ELISA Kit; Neutrophil activating peptide ENA 78 ELISA Kit; Neutrophil activating protein 78 ELISA Kit; Neutrophil-activating peptide ENA-78 ELISA Kit; neutrophil-activating protein 78 ELISA Kit; SCYB5 ELISA Kit; Small inducible cytokine B5 ELISA Kit; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78) ELISA Kit; small inducible cytokine subfamily B; member 5 ELISA Kit; Small-inducible cytokine B5 ELISA Kit
缩写:CXCL5
Uniprot No.:P42830
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:0.625 ng/mL-40 ng/mL
灵敏度:0.567 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 98 | |||||
Range % | 94-103 | ||||||
1:2 | Average % | 102 | |||||
Range % | 97-105 | ||||||
1:4 | Average % | 86 | |||||
Range % | 83-89 | ||||||
1:8 | Average % | 97 | |||||
Range % | 94-100 |
回收率:
The recovery of human ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 85 | 81-89 | |||||
EDTA plasma (n=4) | 98 | 94-103 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected 402.2822.2042.2432.131 201.3621.3681.3651.253 100.7810.7570.7690.657 50.4350.4450.4400.328 2.50.3040.3160.3100.198 1.250.2140.2050.2100.098 0.6250.1880.1840.1860.074 00.1140.1100.112 |
货期:3-5 working days
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