Rat macrophage inflammatory protein 2,MIP-2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E07419r

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
大鼠巨噬细胞炎性蛋白2(MIP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CXCL2含量。CXCL2是重要靶点。它属于趋化因子家族，在炎症反应、肿瘤微环境等中起关键作用。其研究机制主要围绕与受体结合，介导细胞趋化和激活，影响免疫细胞招募、肿瘤细胞迁移与增殖，目前是炎症性疾病和肿瘤治疗研究热点。试剂盒检测范围为78 pg/mL-5000 pg/mL，灵敏度为19.5 pg/mL。适用于科研领域中对炎症机制、药物干预效果及疾病动物模型的研究，例如通过检测组织匀浆中的MIP - 2水平评估局部炎症程度，或分析血液样本中趋化因子动态变化以探索全身性炎症反应的调控通路。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
Cxcl2 ELISA kit; Cinc3 ELISA kit; Mip-2 ELISA kit; Mip2 ELISA kit; Scyb2C-X-C motif chemokine 2 ELISA kit; Cytokine-induced neutrophil chemoattractant 3 ELISA kit; CINC-3 ELISA kit; Macrophage inflammatory protein 2 ELISA kit; MIP2 ELISA kit
缩写：
CXCL2
Uniprot No.：
P30348
种属：
Rattus norvegicus (Rat)
样本类型：
serum, plasma, tissue homogenates
检测范围：
78 pg/mL-5000 pg/mL
灵敏度：
19.5 pg/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Immunology
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-95
1:2	Average %	90
	Range %	85-99
1:4	Average %	98
	Range %	94-101
1:8	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
5000	2.899	2.934	2.916	2.778
2500	2.437	2.567	2.502	2.364
1250	1.817	1.828	1.822	1.684
625	1.228	1.229	1.229	1.091
312	0.736	0.721	0.729	0.591
156	0.445	0.453	0.449	0.311
78	0.263	0.254	0.259	0.121
0	0.141	0.135	0.138